MUSCLE ENERGY PROPERTIES SET STEADY STATE FUNCTION

肌肉能量特性设定稳态功能

• 批准号: 2077466
• 负责人: MICHAEL L BLEI
• 金额: $ 7.77万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2077466
• 关键词: biochemistry; bioenergetics; computer simulation; exercise; human subject; hyperthyroidism; hypothyroidism; laboratory rat; mathematical model; muscle contraction; muscle function; muscle metabolism; noninvasive diagnosis; nuclear magnetic resonance spectroscopy; phosphates; striated muscles

The long term objective of the applicant is to develop an in vivo non- invasive measure that is able to define the functional capacity for sustained activity of human skeletal muscle. The immediate goal is to acquire the necessary skills and knowledge in magnetic resonance spectroscopy experimentation and biochemistry to become a productive independent investigator in the area of cellular energetics. The UCHSC offers a unique environment. It has an established program involving tissue energetics and a Rehabilitation Medicine department whose faculty have a specific clinical interest in the peripheral motor control system and a goal to establish a research program in this area. Laurence Chan, M.D., Ph.D. is the sponsor for this award. Dr. Chan's experience and depth of involvement with in vivo magnetic resonance spectroscopy (MRS) experimentation and knowledge of MRS methodology and tissue energetics will be invaluable. Dr. Chan's sponsorship combined with the support demonstrated by the Department of Rehabilitation Medicine, and the UCHSC will create the foundation for a successful scientific academic career. Phase I: Dr. Chan and the advisory committee have created a two year Research Training Program for the applicant. During the training period the applicant will develop his knowledge in biochemistry and MRS experimentation through a rigorous program. The program includes didactic and independent studies, group seminar participation and computer modelling of cellular energetics. By the completion of the Phase I, the goal is to have a working differential equation based computer model of the energetic process occurring during stimulated isometric contractions within individual fibers with differing fiber-type characteristics. The technical skills and protocols for proton deoxymyoglobin spectroscopy and 31P MRS spectroscopy in the rat hindlimb and human musculature will be developed. Phase II: In the last three years, MRS will be utilized to establish that the skeletal muscle's energetic properties mechanistically determine the maximum steady-state function the muscle can achieve in both normal and pathological conditions. Hyper- and hypothyroid conditions will be used as a model to provide a wide spectrum of muscle energetic profiles in both human anterior tibialis and the rat hindlimb. These conditions will demonstrate the ability to define a spectrum of energetic states, and that both limbs of the energy balance need to be determined to uniquely define the energetics of skeletal muscle.

申请人的长期目标是开发体内非- 侵入性措施，能够定义的功能能力， 人体骨骼肌的持续活动。 近期目标是 获得必要的技能和知识的磁共振 光谱学实验和生物化学成为一个富有成效的 细胞能量学领域的独立研究者 UCHSC 提供了独特的环境。 它有一个既定的计划， 组织能量学和康复医学系， 对外周运动控制系统有特殊的临床兴趣 以及在这一领域建立一个研究项目的目标。 劳伦斯·陈， 医学博士，博士是这个奖项的赞助商。 陈医生的经验及 体内磁共振波谱（MRS）的受累深度 MRS方法学和组织能量学的实验和知识 将是无价的 陈博士的赞助加上 由康复医学系和UCHSC证明 将为成功的科学学术生涯奠定基础。 第一阶段：陈博士和咨询委员会建立了一个为期两年的 申请人的研究培训计划。 培训期间 申请人将发展他在生物化学和MRS方面的知识 通过严格的程序进行实验。 该计划包括 教学和独立研究，小组研讨会的参与， 细胞能量学的计算机建模 在完成 第一阶段，目标是建立一个基于 计算机模型的能量过程中发生的刺激， 具有不同纤维类型的单个纤维内的等长收缩 特色 质子的技术技能和协议 大鼠后肢的脱氧肌红蛋白波谱和31 P MRS波谱 人类的肌肉组织也会发达起来 第二阶段：在最后三年，将利用监测系统， 骨骼肌的能量特性决定了 肌肉在两种正常情况下都能达到的最大稳态功能 和病理条件。 甲状腺功能亢进和甲状腺功能减退的情况将是 用作模型，以提供广泛的肌肉能量分布 在人类胫骨前肌和大鼠后肢中。 这些条件 将展示定义能量状态谱的能力， 并且需要确定能量平衡的两个分支， 唯一地定义了骨骼肌的能量学。