PRESYNAPTIC MECHANISMS OF SOME NEURONAL PLASTICITIES

一些神经元可塑性的突触前机制

• 批准号: 2266961
• 负责人: GEORGE Davis BITTNER
• 金额: $ 14.36万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-12-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2266961
• 关键词: action potentials; alternatives to animals in research; biophysics; calcium channel blockers; calcium flux; crayfish; electrical conductance; electrophysiology; evoked potentials; long term potentiation; magnetic recording system; neural facilitation; neural plasticity; neuropharmacology; neurotransmitter transport; phosphorylation; potassium; potassium channel; sodium; voltage /patch clamp

Our long-term objective is to define electrophysiological and biophysical mechanisms which are responsible for the presynaptic plasticities of facilitation, augmentation, post-tetanic potentiation and long-term potentiation. All of these homosynaptic plasticides will be examined using the crayfish opener excitor neuron whose nerve terminals can be doubly penetrated a fraction of a space constant away from transmitter release sites whose evoked and spontaneous release can be recorded from large, identified postsynaptic muscle cells. Using this preparation, we propose to examine whether increases in calcium currents, decreases in several potassium conductances, and/or increases in internal calcium or sodium concentrations contribute to any or all of these homosynaptic plasticides. Electrophysiological and biophysical paradigms have been carefully designed to measure each of these ionic properties. Given the conservative evolution of many other cellular/molecular mechanisms (including axonal conduction and synaptic transmission), cellular/molecular mechanisms of these synaptic plasticities found at crayfish opener excitor synapses will almost certainly be found at mammalian synapses (including humans). Such knowledge is important because the synaptic plasticities of facilitation, augmentation, and post-tetanic potentiation are probably responsible for such behavioral phenomena as arousal and sensitization, whereas long-term potentiation may be the neuronal basis for learning and memory.

我们的长期目标是定义电生理和生物物理

项目成果

Presynaptic calcium-activated potassium channels and calcium channels at a crayfish neuromuscular junction.

突触前钙激活钾通道和小龙虾神经肌肉接头处的钙通道。

• DOI: 10.1152/jn.1995.73.1.178
• 发表时间: 1995
• 期刊: Journal of neurophysiology.
• 作者: Blundon,JA;Wright,SN;Brodwick,MS;Bittner,GD
• 通讯作者: Bittner,GD