BRAIN PH AND FOCAL CEREBRAL ISCHEMIA

脑PH值和局灶性脑缺血

• 批准号: 2265541
• 负责人: FREDRIC B MEYER
• 金额: $ 15.92万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-15 至 1997-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2265541
• 关键词: acid base balance; blood flow measurement; blood glucose; body temperature; brain imaging /visualization /scanning; brain injury; brain metabolism; cerebral ischemia /hypoxia; histopathology; infarct; laboratory rabbit; nicotinamide adenine dinucleotide; oxygen tension; reperfusion

DESCRIPTION (Investigator's Abstract): The in vivo panoramic imaging system developed during the original grant provides continuous brain PHi, cerebral blood flow, and NADH fluorescence data of the majority of the exposed cortex in the animal model used in this research. This imaging technology has facilitated consistent identification and characterization of the ischemic penumbra, the border zone region of reduced blood flow surrounding the core of dense ischemia or evolving infarction. As described in the Background Data, we have identified acidic foci within the ischemic penumbra. In an evolving infarction, it is postulated that part of the ischemic penumbra deteriorates leading to an increase in the size of the infarction. One of the hypothesis in this grant is that these acidic foci may play a critical role in the recruitment of the ischemic penumbra into infarction. Therefore, comprehending the pathophysiology of these acidic foci may lead to treatments which can reverse this process thereby reducing the degree of ischemic neuronal injury. The experiments outlined in this grant will first study whether the development of the acidic foci is initially due to perfusion or metabolic disturbances. It will then be detained whether enlargement of the infarction is due to expansion and coalescing of the acidic foci or rather expansion of the infarction at the periphery. The remainder of the experiments will examine the effect of various factors on the acidic foci including serum glucose concentrations, P0O2, systemic and extracellular brain pM, reperfusion, and brain temperature.

描述（研究者的摘要）：体内全景成像 原始赠款期间开发的系统提供了连续的大脑 大多数的PHI，脑血流和NADH荧光数据 这项研究中使用的动物模型中暴露的皮质。这 成像技术促进了一致的识别和 缺血性半月的表征，边界区域的边界区域 围绕密度缺血或进化的血液流量减少 梗塞。如背景数据中所述，我们已经确定了 缺血性半体内的酸性灶。在不断发展的梗塞中 假设缺血性半阴茎的一部分会恶化。 增加梗塞的大小。一个假设之一 这项赠款是这些酸性焦点可能在 将缺血性半月招募到梗塞中。所以， 理解这些酸性灶的病理生理可能会导致 可以扭转这一过程的治疗，从而降低学位 缺血性神经元损伤。 该赠款中概述的实验将首先研究 酸性灶的开发最初是由于灌注或代谢 干扰。然后将拘留是否扩大 梗塞是由于酸性焦点的扩张和聚集 而不是外围梗塞的扩展。其余的 实验将检查各种因素对酸性的影响 焦点包括血清葡萄糖浓度，P0O2，全身和 细胞外脑PM，再灌注和大脑温度。