UNIVERSITY OF LOUISVILLE NIEHS CENTER DEVELOPMENTAL GRAN

一项未来的工业健康监测计划已经得到协调 作者：卡洛·H·坦布罗，医学博士，M.P.H.，职业毒理学分部， 美国路易斯维尔大学医学院在过去的20年里。本节目 提供一组独特的人类医疗数据、工作接触史和 适合短期和长期分析的组织/血清样本 接触有毒化学物质的生物标志物。这一职业的曝光 对工业化学品的人口进行持续监测，并 验证周期为12-40年。在大路易斯维尔地区， 大量医疗不应得和经济上处于不利地位的人口 居住在工业现场附近，他们的病史可以 可以与工业同龄人相比。这些劣势住宅 可以通过拟议的生物标记物计划来监测种群，以评估 相对于产业工人和他们的同龄人，他们的身体负担。 该中心的医疗监测方面将提供必要的 人类医疗数据、工作历史和组织以发展相关性 建议的短期和长期生物标记物与实际 与这些化学排放物相关的疾病过程 工业场地。获得的数据将允许更可靠的因果关系 关于人口和 验证生物标志物的敏感性和特异性。本次大会的主题是 L中心的U将重点介绍生化、化学和生物化学的使用 用于确定人群暴露的分子生物学标志物 并在这些受试者中评估随后的健康风险 预防和治疗方式。 已经选定了五个试点项目，这些项目将侧重于 路易斯维尔工业和居住人口的监测 暴露于丙烯腈、氯乙烯和各种金属 具有已知毒理学重要性的催化剂。项目1(坦布罗/鲁尼) 涉及医疗数据和暴露的收集、存储和分析 人类组织样本的历史和收集将在 以下项目的生物标记物计划。项目2(奔驰/梦幻岛) 将研究丙烯腈和氯乙烯在大鼠体内的代谢 人肝细胞。项目3(赫斯特/迈尔斯)将评估生物标记物 接触血红蛋白和DNA加合物的中间持续时间 丙烯腈的含量。项目4(布伦南/普罗)将进行简短和 可能是丙烯腈、氯乙烯、 和金属催化剂暴露，即诱导和持久 外源化合物代谢的几种酶的表达。项目 5(Geoghegan/Myers/Wong)将评估一个长期生物标记物，即 已知与癌症相关的P53抑癌基因突变 血管肉瘤源于人体暴露于丙烯腈。

A prospective industrial health surveillance program has been coordinated by Carlo H. Tamburro, M.D., M.P.H., Division of Occupational Toxicology, U. Louisville School of Medicine for the past 20 years. This program provides a unique set of human medical data, work-exposure histories and tissue/serum samples suitable for analysis of short-and long-term biomarkers of toxic chemical exposure. The exposure of this occupational population to industrial chemicals has been continuously monitored and verified for periods of 12-40 years. In the Greater Louisville area, a large medically undeserved and economically-disadvantaged population resides adjacent to the industrial sites and their medical histories can be compared to industrial cohorts. These disadvantage residential populations can be monitored by the proposed biomarker program to assess their body burden relative to the industrial workers and their cohorts. The medical surveillance aspects of this Center will provide essential human medical data, work histories and tissue to develop correlations between the proposed short and long term-biomarkers with the actual disease processes associated with the chemical effluent of these industrial sites. The data obtained would allow more reliable causal linkages to be made or disprove with regard to the populations and validate the biomarkers sensitivity and specificity. The Theme for the U of L Center will focus on the use of biochemical, chemical, and molecular biological biomarkers to define exposure in human populations and to assess in these subjects subsequent health risk requiring preventive and therapeutic modalities. Five Pilot Projects have been selected which would focus on the health surveillance of industrial and residential populations in Louisville exposures to acrylonitrile, vinyl chloride, and a variety of metal catalysts of known toxicological importance. Project 1 (Tamburro/Looney) involves collection, storage, and analysis of medical data & exposure histories and collection of human tissue samples to be analyzed in the biomarker program of the following Projects. Project 2 (Benz/Nerland) will study metabolism of acrylonitrile and vinyl chloride in rat and human hepatocytes. Project 3 (Hurst/Myers) will evaluated biomarkers of intermediate duration of exposure involving hemoglobin and DNA adducts of acrylonitrile. Project 4 (Brennan/Prough) will evaluated short and potentially intermediate-term markers of acrylonitrile, vinyl chloride, and metal catalyst exposure, namely induction and persistence of expression of several enzymes of foreign compound metabolism. Project 5 (Geoghegan/Myers/Wong) will evaluated a long term biomarker, namely mutation of p53 tumor suppressor gene known to be associated with angiosarcomas derived from acrylonitrile exposure in humans.