ELECTROPHYSIOLOGY OF RETROHIPPOCAMPAL NEURONS

海马后神经元的电生理学

• 批准号: 2271741
• 负责人: MARK G STEWART
• 金额: $ 12.11万
• 依托单位: SUNY DOWNSTATE MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2271741
• 关键词: antidromic impulse; central neural pathway /tract; dyes; electrophysiology; electrostimulus; entorhinal cortex; gamma aminobutyrate; glutamates; hippocampus; laboratory rat; limbic system; membrane potentials; micromanipulator; neural information processing; neurons; neurophysiology

The hippocampal formation can be divided into two circuits of neurons on the basis of anatomical connections and the behavioral correlates of firing of cells. The "Ammon's horn circuit" is an essentially unidirectional loop of excitatory neurons from the entorhinal cortex (EC) through the dentate gyrus, Ammon's horn (the so-called tri-synaptic pathway), and subiculum. Entorhinal, Ammon's horn and subicular neurons are known to fire action potentials when the animal is in a particular location in the recording environment (place cells). In those parts of the "presubicular circuit" that have been examined (presubiculum, postsubiculum, some anterior thalamic nuclei and retrosplenial cortex), neurons fire action potentials when the animal faces a particular direction within the recording environment (head direction cells). This project will examine the electrophysiology of three retrohippocampal regions: a) the pre and postsubiculum (members of the head direction cell circuit); b) the subiculum (member of the place cell circuit); and c) the entorhinal Cortex (the structure that controls information flow between the two circuits). The electrophysiological properties of single neurons, their local connectivity and their contributions to the pre-/postsubiculum to entorhinal to subiculum pathway will be studied in slices and in anesthetized animals. The purpose will be to: a) characterize the cells of the electrophysiologically undefined subicular complex; b) identify the specific neuronal elements in the disynaptic pathway that serves to transfer information between two distinct limbic system circuits; and c) to provide electrophysiological criteria for interpreting recordings from other preparations such as extracellular unit data from freely behaving animals. This work is important in the understanding of navigation, a function that is frequently lost in dementias. Also, because all output from the hippocampal formation goes through the subiculum or the entorhinal cortex, this work will be important in the understanding of the spread of epileptic activity to other parts of brain.

海马结构可分为两个神经元回路 解剖学联系的基础和行为相关性 细胞放电。 “阿蒙号角电路”本质上是一个 内嗅皮层 (EC) 兴奋性神经元的单向环 通过齿状回、阿蒙角（所谓的三突触 途径）和下托。内嗅神经元、阿蒙角神经元和皮下神经元 已知当动物处于特定状态时会激发动作电位 记录环境中的位置（位置单元）。在那些部分 已经检查过的“下前回路”（presubiculum， 下潜，一些前丘脑核和压后皮质）， 当动物面对特定的情况时，神经元会激发动作电位 记录环境内的方向（头部方向单元）。 该项目将检查三个海马后的电生理学 区域：a) 前枕骨和后枕骨（头部方向细胞的成员） 电路）; b) 下托（位置细胞回路的成员）； c） 内嗅皮层（控制信息流的结构 两个电路）。 单个神经元的电生理特性及其局部 连接性及其对前/后subbiculum的贡献 内嗅到下托的途径将在切片和中进行研究 麻醉的动物。目的是： a) 表征细胞 电生理学上未定义的皮下复合体； b) 确定 突触通路中的特定神经元元件 在两个不同的边缘系统回路之间传输信息；和c) 提供电生理学标准来解释记录 其他准备工作，例如来自自由行为的细胞外单位数据 动物。 这项工作对于理解导航非常重要，导航是一种功能 经常因痴呆症而丧失。另外，因为所有输出 海马结构穿过下托或内嗅皮层， 这项工作对于理解病毒的传播非常重要 癫痫活动对大脑其他部分的影响。