RETROHIPPOCAMPAL CIRCUITS MEDIATED EPILEPTIFORM ACTIVITY

描述：(改编自《调查者摘要》)：内嗅皮层 可以产生独立于海马体的癫痫样活动，它可以 维持在海马体中产生的癫痫样活动，它就会开启 这种活动扩散到新大脑皮层。虽然有密集的工作 CA3区癫痫样活动的基础及一些现象学研究 在内嗅觉和海马区惊厥发生器的相互作用中，有 几乎没有人注意到海马后部 区域本身就会产生癫痫发作活动。造成这种情况的部分原因是 一直缺乏关于基本电路和蜂窝属性的数据 海马区后细胞。我们的实验室一直在研究细胞和电路 海马后区，包括内嗅皮层、丘脑前、 副下丘和下丘。 这个项目的目的是定义电池和内部电路 内嗅皮层及其在癫痫样形成中的作用 出院。我们的总体假设是癫痫样事件(发作间歇期 棘波和后放电)在内嗅皮层中由 未发现椎板内和椎板间兴奋性连接的组合 在其他海马区或海马区结构中。单个神经元将成为 电生理和形态特征。的属性 单一和复合兴奋性突触后电位将在 已识别的细胞。已鉴定神经元的细胞和突触特性 将与它们在癫痫样放电过程中的活动相关 无拘无束的切片。 这些结果将提供有关中单元格基本属性的基本数据 内嗅皮层及其内在的连通性。这些数据将成为 了解老年人正常生理和异常生理的基础 海马后区。这些细胞的特性和它们形成的电路 在一种癫痫模型中(在解除抑制过程中所见)将显示出一些 电路的功能属性。这些功能属性将 对癫痫的发生和癫痫的传播提供重要的见解 内嗅皮层的活动。对这类电路的了解可能会导致 到限制癫痫的产生和传播的实用策略 断线程序。

DESCRIPTION: (Adapted from the Investigator's Abstract): The entorhinal cortex can generate epileptiform activity independent of the hippocampus, it can sustain epileptiform activity generated in the hippocampus, and it gates the spread of such activity to the neocortex. While there has been intensive work on the basis for epileptiform activity in CA3 and some phenomenological studies of the interactions of entorhinal and hippocampal seizure generators, there has been virtually no attention paid to the mechanism by which the retrohippocampal areas generate seizure activity themselves. Part of the reason for this has been a lack of data on the fundamental circuitry and cellular properties of retrohippocampal cells. Our laboratory has been studying the cells and circuits of the retrohippocampal regions, including entorhinal cortex, presubiculum, parasubiculum and subiculum. The aim of this project is to define the cells and intrinsic circuits of the entorhinal cortex and to identify their roles in the generation of epileptiform discharges. Our overall hypothesis is that epileptiform events (interictal spikes and after discharges) are generated in entorhinal cortex by a combination of intralaminar and interlaminar excitatory connections not found in other retrohippocampal or hippocampal structures. Single neurons will be electrophysiologically and morphologically characterized. The properties of unitary and compound excitatory postsynaptic potentials will be examined in identified cells. The cellular and synaptic properties of identified neurons will be correlated with their activity during epileptiform discharges in disinhibited slices. The results will provide fundamental data on the basic properties of cells in the entorhinal cortex and its intrinsic connectivity. These data will be part of the foundation for understanding the normal and abnormal physiology of the retrohippocampal regions. The properties of these cells and circuits they form in one model of epilepsy (that seen during disinhibition) will demonstrate some of the functional properties of the circuitry. These functional properties will provide critical insights into epileptogenesis and the spread of seizure activity in the entorhinal cortex. An understanding of such circuits may lead to practical strategies for limiting seizure generation and spread by targeted disconnection procedures.