T-CELL RECEPTORS INVOLVED IN TMEV-INDUCED DEMYELINATION

说明(改编自申请人的摘要)： 本申请中描述的研究是为了检查 TMEV特异性TCR谱带的差异之间的相关性 以及TMEV诱导的脱髓鞘的进展。四个具体目标 建议：1)通过TMEV特定和非TMEV来确定VB和JB的使用情况 活动性脱髓鞘过程中特异性中枢神经系统T细胞的浸润。2)至 脑感染后中枢神经系统T细胞与病原性T细胞的比较 和低致病性TMEV.3)评估TCR谱系的变化 T细胞在病程改变后的渗入 TMEV表位免疫。4)分析潜在的影响 H-2DS易感基因座和J-2DD抗性基因座在TCR选择中的作用 针对TMEV抗原的曲目。无论是免疫学还是分子生物学 将采用生物学方法来实现 这个项目的目标。

DESCRIPTION (Adapted from the Applicant's Abstract): The purpose of the study described in this application is to examine the potential correlation between the difference in the TMEV-specific TCR repertoire and the development of TMEV-induced demyelination. Four specific aims are proposed:1) To determine Vb and Jb usage by TMEV-specific and non specific CNS-infiltrating T cells during active demyelination. 2) To compare the T cells in CNS after intracerebral infection with pathogenic and low-pathogenic TMEV.3) To assess changes in the TCR repertoire of infiltrating T cells after alteration of the disease course by immunization with TMEV epitopes. 4) To analyze potential effects of susceptible H-2Ds and resistant J-2Dd loci on the selection of the TCR repertoire specific for TMEV antigens. Both immunological and molecular biological approaches will be employed towards achievement of the objectives of this project.