T-CELL RECEPTORS INVOLVED IN TMEV-INDUCED DEMYELINATION

描述（改编自申请人的摘要）： 本申请中描述的研究是为了检查 TMEV特异性TCR库的差异与TMEV特异性TCR库的差异之间的相关性 以及TMEV诱导的脱髓鞘的发展。 四个具体目标 1）通过TMEV特异性和非特异性的TMEV来确定Vb和Jb的使用 特异性CNS浸润性T细胞。 2)到 比较脑内感染后CNS中的T细胞与致病性 3）为了评估TMEV的TCR库的变化， 浸润性T细胞改变后的疾病进程， 用TMEV表位免疫。 4)分析潜在影响 敏感H-2Ds和抗性J-2Dd基因座对TCR选择的影响 TMEV抗原的特异性库。 免疫学和分子生物学 生物学方法将用于实现 这个项目的目标。

DESCRIPTION (Adapted from the Applicant's Abstract): The purpose of the study described in this application is to examine the potential correlation between the difference in the TMEV-specific TCR repertoire and the development of TMEV-induced demyelination. Four specific aims are proposed:1) To determine Vb and Jb usage by TMEV-specific and non specific CNS-infiltrating T cells during active demyelination. 2) To compare the T cells in CNS after intracerebral infection with pathogenic and low-pathogenic TMEV.3) To assess changes in the TCR repertoire of infiltrating T cells after alteration of the disease course by immunization with TMEV epitopes. 4) To analyze potential effects of susceptible H-2Ds and resistant J-2Dd loci on the selection of the TCR repertoire specific for TMEV antigens. Both immunological and molecular biological approaches will be employed towards achievement of the objectives of this project.