T-CELL RECEPTORS INVOLVED IN TMEV-INDUCED DEMYELINATION

T 细胞受体参与 TMEV 诱导的脱髓鞘

• 批准号: 2271543
• 负责人: BYUNG S KIM
• 金额: $ 15.44万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 1998-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2271543
• 关键词: T cell receptor; helper T lymphocyte; histocompatibility antigens; laboratory mouse; leukocyte activation /transformation; microorganism immunology; murine encephalomyelitis virus; polymerase chain reaction; protein sequence; suppressor T lymphocyte; tissue /cell culture; viral myelinopathy

DESCRIPTION (Adapted from the Applicant's Abstract): The purpose of the study described in this application is to examine the potential correlation between the difference in the TMEV-specific TCR repertoire and the development of TMEV-induced demyelination. Four specific aims are proposed:1) To determine Vb and Jb usage by TMEV-specific and non specific CNS-infiltrating T cells during active demyelination. 2) To compare the T cells in CNS after intracerebral infection with pathogenic and low-pathogenic TMEV.3) To assess changes in the TCR repertoire of infiltrating T cells after alteration of the disease course by immunization with TMEV epitopes. 4) To analyze potential effects of susceptible H-2Ds and resistant J-2Dd loci on the selection of the TCR repertoire specific for TMEV antigens. Both immunological and molecular biological approaches will be employed towards achievement of the objectives of this project.

说明(改编自申请人的摘要)： 本申请中描述的研究是为了检查 TMEV特异性TCR谱带的差异之间的相关性 以及TMEV诱导的脱髓鞘的进展。四个具体目标 建议：1)通过TMEV特定和非TMEV来确定VB和JB的使用情况 活动性脱髓鞘过程中特异性中枢神经系统T细胞的浸润。2)至 脑感染后中枢神经系统T细胞与病原性T细胞的比较 和低致病性TMEV.3)评估TCR谱系的变化 T细胞在病程改变后的渗入 TMEV表位免疫。4)分析潜在的影响 H-2DS易感基因座和J-2DD抗性基因座在TCR选择中的作用 针对TMEV抗原的曲目。无论是免疫学还是分子生物学 将采用生物学方法来实现 这个项目的目标。