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ON-SITE ENUMERATION OF FUNGAL SPORES AND BACTERIA IN AIR

空气中真菌孢子和细菌的现场计数

基本信息

批准号：
2277621
负责人：
H K DILLON
金额：
$ 14.26万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
1992
资助国家：
美国
起止时间：
1992-02-01 至 1996-01-31
项目状态：
已结题

项目摘要

The overall objective of the proposed work is to develop an on-site screening method for the enumeration of fungal spores and bacteria in air samples. It is proposed that air sampling be carried out with a size- selective device upstream form an appropriate membrane filter disc with subsequent analysis by a combination of visible and fluorescent microscopy. The primary goal of the analytical methods development effort will be to produce an analysis protocol that can be used in the field and that will yield results within minutes of the cessation of air sampling. Enumeration of fungal spores and bacteria will be accomplished by supravital staining organisms with fluorescent probes and then observing them by epifluorescence microscopy and, if possible, by transmitted light microscopy. The air sampling device will be fabricated to collect primarily thoracic particles (i.e., those particles that pass beyond the head and neck during breathing). Particles will be deposited on a membrane filter that is found to be suitable for microscopic analysis. Test atmospheres of a nonbiological fluorescent aerosol and representative fungal spores will be produced in the laboratory for the evaluation of the sampling and analytical procedure developed. A field evaluation of the effectiveness of the procedure in assessing the potential exposure to airborne biological aerosols that have been associated with increased incidence of pulmonary disease will be undertaken. Total and aggregate counts of fungal spores and bacteria will be compared to results obtained by scanning electron microscopy. Count concentrations will also be compared to results of the culturing of impactor samples. Time/concentration profiles will be generated in a variety of agricultural environments and in an office building.

拟议工作的总体目标是制定一个现场空气中真菌孢子和细菌计数的筛选方法样品建议进行空气取样，取样尺寸为- 选择性装置上游形成适当的膜过滤盘，随后通过可见光和荧光显微镜的组合进行分析。分析方法开发工作的主要目标是制定一个可以在现场使用的分析协议，在停止空气采样后数分钟内得出结果。枚举真菌孢子和细菌的鉴定将通过超活体染色来完成然后用荧光探针观察它们，落射荧光显微镜，如果可能，通过透射光显微镜空气采样装置将被制作成收集主要是胸腔颗粒（即，这些粒子穿过呼吸时的头部和颈部）。微粒会沉积在膜上过滤器被认为是适合于显微镜分析。测试非生物荧光气溶胶和代表性的大气真菌孢子将在实验室中产生，用于评估制定了取样和分析程序。实地评价评估潜在接触的程序的有效性空气中的生物气溶胶，肺疾病的发病率将进行。共计和合计将真菌孢子和细菌计数与获得的结果进行比较通过扫描电子显微镜。计数浓度也将与撞击器样品的培养结果相比。时间/浓度分布图将在各种农业环境和办公楼。