ON-SITE ENUMERATION OF FUNGAL SPORES AND BACTERIA IN AIR

空气中真菌孢子和细菌的现场计数

基本信息

批准号：
2277621
负责人：
H K DILLON
金额：
$ 14.26万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
1992
资助国家：
美国
起止时间：
1992-02-01 至 1996-01-31
项目状态：
已结题

项目摘要

The overall objective of the proposed work is to develop an on-site screening method for the enumeration of fungal spores and bacteria in air samples. It is proposed that air sampling be carried out with a size- selective device upstream form an appropriate membrane filter disc with subsequent analysis by a combination of visible and fluorescent microscopy. The primary goal of the analytical methods development effort will be to produce an analysis protocol that can be used in the field and that will yield results within minutes of the cessation of air sampling. Enumeration of fungal spores and bacteria will be accomplished by supravital staining organisms with fluorescent probes and then observing them by epifluorescence microscopy and, if possible, by transmitted light microscopy. The air sampling device will be fabricated to collect primarily thoracic particles (i.e., those particles that pass beyond the head and neck during breathing). Particles will be deposited on a membrane filter that is found to be suitable for microscopic analysis. Test atmospheres of a nonbiological fluorescent aerosol and representative fungal spores will be produced in the laboratory for the evaluation of the sampling and analytical procedure developed. A field evaluation of the effectiveness of the procedure in assessing the potential exposure to airborne biological aerosols that have been associated with increased incidence of pulmonary disease will be undertaken. Total and aggregate counts of fungal spores and bacteria will be compared to results obtained by scanning electron microscopy. Count concentrations will also be compared to results of the culturing of impactor samples. Time/concentration profiles will be generated in a variety of agricultural environments and in an office building.

拟议工作的总体目标是开发现场用于枚举真菌孢子和空气中细菌的筛查方法样品。建议用大小 - 进行空气采样选择性设备上游形成适当的膜过滤盘，随后通过可见光和荧光显微镜组合进行分析。分析方法开发工作的主要目标是生成可以在现场使用的分析协议，并将在停止空气采样后的几分钟内产生产量。枚举真菌孢子和细菌将通过胸膜染色来完成荧光探针的生物，然后通过表现显微镜，如果可能的话，显微镜。空气采样设备将被制造以收集主要是胸腔颗粒（即那些超出了超越的粒子呼吸过程中的头部和颈部）。颗粒将沉积在膜上发现适合微观分析的过滤器。测试非生物荧光气溶胶和代表性的大气真菌孢子将在实验室生产以评估制定了采样和分析程序。对该程序评估潜在暴露的有效性与增加有关的机载生物气溶胶将发生肺部疾病的发病率。总和将真菌孢子和细菌的计数与获得的结果进行比较通过扫描电子显微镜。计数浓度也将是与影响器样品培养的结果相比。时间/浓度概况将在各种农业中产生环境和办公大楼。