ON-SITE ENUMERATION OF FUNGAL SPORES AND BACTERIA IN AIR

空气中真菌孢子和细菌的现场计数

• 批准号: 3420977
• 负责人: H K DILLON
• 金额: $ 11.92万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-02-01 至 1995-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3420977
• 关键词: Fungi; acridines; aerosols; agriculture; air filtration; air sampling /monitoring; analytical method; bacteria; endotoxins; environmental contamination; field study; fluorescence microscopy; fluorescent dye /probe; light microscopy; microorganism culture; morphology; occupational health /safety; scanning electron microscopy; spores; statistics /biometry; systematic biology

The overall objective of the proposed work is to develop an on-site screening method for the enumeration of fungal spores and bacteria in air samples. It is proposed that air sampling be carried out with a size- selective device upstream form an appropriate membrane filter disc with subsequent analysis by a combination of visible and fluorescent microscopy. The primary goal of the analytical methods development effort will be to produce an analysis protocol that can be used in the field and that will yield results within minutes of the cessation of air sampling. Enumeration of fungal spores and bacteria will be accomplished by supravital staining organisms with fluorescent probes and then observing them by epifluorescence microscopy and, if possible, by transmitted light microscopy. The air sampling device will be fabricated to collect primarily thoracic particles (i.e., those particles that pass beyond the head and neck during breathing). Particles will be deposited on a membrane filter that is found to be suitable for microscopic analysis. Test atmospheres of a nonbiological fluorescent aerosol and representative fungal spores will be produced in the laboratory for the evaluation of the sampling and analytical procedure developed. A field evaluation of the effectiveness of the procedure in assessing the potential exposure to airborne biological aerosols that have been associated with increased incidence of pulmonary disease will be undertaken. Total and aggregate counts of fungal spores and bacteria will be compared to results obtained by scanning electron microscopy. Count concentrations will also be compared to results of the culturing of impactor samples. Time/concentration profiles will be generated in a variety of agricultural environments and in an office building.

拟议工作的总体目标是开发一个现场 空气中真菌孢子和细菌计数的筛选方法 样品。 建议采用以下尺寸进行空气采样： 选择装置上游形成适当的膜过滤盘 随后通过可见光显微镜和荧光显微镜相结合进行分析。 分析方法开发工作的主要目标是 制定可在现场使用的分析方案 空气采样停止后几分钟内即可得出结果。 枚举 真菌孢子和细菌的检测将通过超活体染色来完成 用荧光探针观察生物体，然后通过 落射荧光显微镜，如果可能的话，通过透射光 显微镜。 空气采样装置将被制造来收集 主要是胸腔颗粒（即那些超出胸腔的颗粒） 呼吸时头部和颈部）。 颗粒将沉积在膜上 发现适合显微分析的过滤器。 测试 非生物荧光气溶胶的气氛和代表性 真菌孢子将在实验室中产生，用于评估 制定了取样和分析程序。 实地评估 评估潜在暴露的程序的有效性 空气中的生物气溶胶与增加有关 将进行肺部疾病的发生率。 总计和总计 真菌孢子和细菌的计数将与获得的结果进行比较 通过扫描电子显微镜。 计数浓度也将是 与撞击样品的培养结果进行比较。 将在各种农业中生成时间/浓度曲线 环境和办公楼中。