MAPPING AND CHARACTERIZATION OF AGE MUTANTS

年龄突变体的绘图和表征

• 批准号: 2055547
• 负责人: Stacey A Duhon
• 金额: $ 1.93万
• 依托单位: UNIVERSITY OF COLORADO
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-30 至 1997-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2055547
• 关键词: Caenorhabditis elegans; SDS polyacrylamide gel electrophoresis; aging; alternatives to animals in research; animal breeding; fertility; gene complementation; gene induction /repression; genetic mapping; genetic strain; hermaphroditism; hydrogen peroxide; invertebrate embryology; invertebrate locomotion; mutant; oxidative stress; phenotype; polymerase chain reaction; radiation resistance; stress proteins; thermostability; ultraviolet radiation; western blottings

The standing long-term goal of this research direction has been to understand the molecular and physiological processes that lead to aging in the nematode Caenorhabditis elegans. I have found three new alleles of age-1 which is a gene that extends life span. We propose to identify the molecular and physiological bases of these life-prolongation effects and to directly test the hypothesis that prolongation of life span results from the enhancement of processes mediating the ability to withstand toxic stress. My aims are to (A) finish backcrosses, complemenation tests and mapping of the newly isolated Age mutants; (B) test for the stress- resistant phenotypes (thermotolerance, UV resistance, and H2O2 resistance) of each of these strains; (C) if mutants show increased thermotolerance, determine whether hsp-16 is more readily induced, as is the case for age- 1; and (D) assess behavior, development and other life history traits on the new Age mutants.

这一研究方向的长期目标是 了解导致衰老的分子和生理过程 线虫。我发现了三个新的等位基因 AGE-1，这是一种延长寿命的基因。我们建议确定 这些延寿作用的分子和生理基础以及 直接检验延长寿命会带来寿命延长的假设 从增强调节毒物抵抗能力的过程 压力。我的目标是(A)完成回交、补全测试和 新分离的年龄突变体的作图；(B)压力测试- 抗性表型(耐热性、抗紫外线和抗过氧化氢) 这些菌株中的每一个；(C)如果突变株表现出更高的耐热性， 确定是否更容易诱发HSP-16，就像年龄- 1；和(D)评估行为、发育和其他生活史特征 新时代的变种人。