FLOW CYTOMETRIC ANALYSIS OF MULTIPLE DNA FLUOROCHROMES

多种 DNA 荧光染料的流式细胞术分析

• 批准号: 2283287
• 负责人: HARRY A CRISSMAN
• 金额: $ 35.69万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-01 至 1997-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2283287
• 关键词: CHO cells; DNA; bioengineering /biomedical engineering; cell cycle; cell differentiation; cell population study; chemical binding; chemical kinetics; chromatin; computer data analysis; fibroblasts; flow cytometry; fluorescence spectrometry; fluorescent dye /probe; histochemistry /cytochemistry; interferometry; laser spectrometry; linear energy transfer; molecular rearrangement; nucleic acid structure; nucleobase; nucleoproteins; stainings; synchronous cell division

Flow cytometric (FCM) analyses of the differential in DNA-binding by multiple base-specific fluorochromes can provide a unique approach for detecting metabolic changes in the native state of chromatin in individual cells responding to changes in the physiological state of the population. This rationale is based on the premise that subtle rearrangements in chromatin organization involve modulations in DNA- nucleoprotein interactions resulting in disproportionate changes in DNA accessibility to base-specific fluorochromes that can be efficiently detected by FCM. Such new technology provides distinct advantages for clinical, structural, and cell biology studies including, (a) the application of DNA fluorochromes to cells or chromosomes under relatively non-perturbing conditions so that chromatin remains close to the native configuration, (b) rapid FCM analyses for correlating the relative changes in binding by multiple fluorochromes in cells and (c) requirement of small numbers of cells. The long term goal is to develop this new FCM approach to increase the sensitivity for detecting cell cycle-related changes in chromatin organization and for identifying individual chromosomes. Achieving this goal will require (1) detailed spectral studies on selected, DNA based-specific fluorochromes bound to DNA and chromatin, (2) utilizing unique FCM spectroscopic and conventional instrumentation to quantitate relative changes in binding features of multiple DNA fluorochromes to chromatin in cells and chromosomes, (3) applying new computer analyses methods that can better correlate binding by multiple fluorochromes. Specific Aim 1 is perform spectrofluorometric studies on DNA-specific dyes, including new, ultrasensitive fluorochromes, such as TOTO and YOYO, to determine fluorescence lifetime, energy transfer, and other dye-dye interactions that will provide the rationale for developing multifluorochrome labeling techniques. Specific Aim 2 is to evaluate the sensitivity of unique analytical approaches utilizing recently developed FCM instrumentation, including our phase sensitive detection FCM, the Fourier transform FCM, as well as our multiparameter three laser FCM system and the two laser, chromosome analysis FCM. Specific Aim 3 is to apply new computer analysis methods, including the IDYLK analysis programs, cluster analysis, differential fluorescence and ratio analyses as developed in this laboratory to correlate differential changes in DNA accessibility to base-specific fluorochromes with the cell cycle-related modifications of nucleoproteins to obtain a better understanding of the molecular basis for the alterations in the cytochemical patterns of DNA accessibility.

流式细胞术（FCM）分析DNA结合的差异， 多种碱基特异性荧光染料可以提供一种独特的方法， 检测染色质天然状态的代谢变化， 个体细胞响应于细胞的生理状态的变化， 人口 这一基本原理是基于这样一个前提， 染色质组织的重排涉及DNA的调节， 核蛋白相互作用导致DNA不成比例的变化 可获得的碱基特异性荧光染料， FCM检测。 这种新技术为以下方面提供了明显的优势： 临床、结构和细胞生物学研究，包括（a） 将DNA荧光染料应用于细胞或染色体， 非干扰条件，使染色质保持接近天然 （B）快速FCM分析，用于关联相对 细胞中多种荧光染料结合的变化和（c）要求 少量的细胞。 长期目标是开发这种新的FCM 增加检测细胞周期相关的敏感性的方法 染色质组织的变化，并用于识别个体 染色体 实现这一目标将需要（1）详细的光谱 研究选定的，基于DNA的特异性荧光染料结合到DNA和 染色质，（2）利用独特的FCM光谱和常规 仪器，以定量结合特征的相对变化， 细胞和染色体中的染色质的多种DNA荧光染料，（3） 应用新的计算机分析方法， 通过多种荧光染料。 具体目标1是进行荧光分光光度法 DNA特异性染料的研究，包括新的、超灵敏的 荧光染料，如TOTO和YOYO，以确定荧光寿命， 能量转移和其他染料-染料相互作用， 开发多荧光染料标记技术的基本原理。 具体 目的2是评估独特分析方法的灵敏度 利用最近开发的FCM仪器，包括我们的阶段 灵敏检测FCM，傅里叶变换FCM，以及我们的 多参数三激光FCM系统和双激光、染色体 FCM分析。 具体目标3是应用新的计算机分析方法， 包括IDYLK分析程序，聚类分析，差异分析， 本实验室开发的荧光和比率分析， 将DNA可及性的差异变化与碱基特异性 具有核蛋白的细胞周期相关修饰的荧光染料 为了更好地了解 DNA可及性的细胞化学模式的改变。