CYTOKINES--THEIR ROLE IN COXSACKIE VIRUS B3 MYOCARDITIS

本拨款申请中讨论的研究涉及以下方面的作用 细胞因子在病毒性心肌炎急性期和晚期发展中的作用 CB 3感染的易感A/J小鼠的阶段自身免疫性心肌炎， 人柯萨奇病毒性心肌炎模型。实验将检查当地 响应于CB 3感染在心脏中产生细胞因子，或 肌球蛋白免疫细胞因子对炎症细胞的影响 人群及其活动，以及细胞因子对心脏 将检查肌细胞收缩性和生理学。的影响 抗细胞因子治疗对心肌炎的发展也将是 考察 所有研究将在约翰霍普金斯卫生学院进行， 公共卫生实验室设备齐全，包含所有 完成所述实验所需的设备。

The research discussed in this grant proposal addresses the role of cytokines in the development of acute phase viral myocarditis and late phase autoimmune myocarditis in CB3- infected susceptible A/J mice, a model of human coxsackievirus myocarditis. Experiments will examine local production of cytokines in the heart in response to CB3 infection or myosin immunization. The effects of cytokines on inflammatory cell populations and their activity, and the effects of cytokines on cardiac myocyte contractility and physiology will be examined. The effect of anticytokine therapy on the development of myocarditis will also be examined. All research will be conducted in the Johns Hopkins School of Hygiene and Public Health. The laboratories are well equipped, and contain all equipment necessary to complete the described experiments.