REGULATION OF NEUROPEPTIDE BIOSYNTHESIS AND SECRETION

神经肽生物合成和分泌的调节

• 批准号: 2259633
• 负责人: VICTOR MAY
• 金额: $ 7.06万
• 依托单位: UNIVERSITY OF VERMONT & ST AGRIC COLLEGE
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-12-15 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2259633
• 关键词: ascorbate; calcium flux; catecholamines; cell cell interaction; gene induction /repression; glucocorticoids; immunocytochemistry; immunoelectron microscopy; in situ hybridization; laboratory rat; mixed tissue /cell culture; neural plasticity; neuropeptide Y; neuropeptide receptor; neuropharmacology; neurotransmitter biosynthesis; norepinephrine; northern blottings; phenotype; second messengers; secretion; superior cervical ganglion

The goal of this study is to determine the central regulators of neuronal function using cultured superior cervical ganglion (SCG) neurons as models. The focus of this proposal is to examine the regulatory interrelationship between neuronal peptide and classical transmitter expression, and in particular, the expression of neuropeptide Y (NPY) and catecholamines (CA) in the SCG. Several questions examining the factors and environmental cues that coordinately or independently regulate SCG NPY and catecholamine (CA) expression will be addressed, including (1) What are the regulators of neuronal NPY expression? (2) Are NPY and CA expression altered by the same regulatory factors? (3) How does neuronal phenotype affect NPY expression? (4) Do specific target tissues regulate neuronal NPY expression? (5) What are the cellular mechanisms of altered neuropeptide expression? Several parameters will be used to characterize culture NPY/CA content and biosynthesis including radioimmunoassay, biosynthetic labeling techniques, Northern blot analysis, and HPLC/electrochemical detection techniques. The effects of neuronal input onto SCG cells, that is stimulation of second messenger system, calcium flux, depolarization, and specific receptor- mediated stimulation on SCG NPY production will be examined and correlated to CA production levels. How factors that change SCG neurotransmitter expression from catecholaminergic to cholinergic phenotype alter NPY expression will be examined. In coculture studies, the effects of SCG target cells on neurotransmitter/neuropeptide expression will be determined. These studies will have significant implications in establishing how complex and integrated signals direct normal neuronal development and physiological functions.

本研究的目的是确定神经元的中枢调节因子。 以培养的颈上神经节(SCG)神经元为模型。 这项提案的重点是审查监管之间的相互关系 在神经肽和经典递质表达之间，以及在 特别是神经肽Y(NPY)和儿茶酚胺(CA)的表达 在SCG里。考察因素和环境线索的几个问题 协同或独立调节SCG、NPY和儿茶酚胺(CA) 将解决的表达，包括(1)什么是监管机构 神经细胞NPY的表达？(2)NPY和CA的表达是否受同样的影响 调控因素？(3)神经细胞表型如何影响NPY的表达？ (4)特定的靶组织是否调节神经元NPY的表达？(5)什么 神经肽表达改变的细胞机制是什么？几个 参数将用于表征培养NPY/CA含量和 生物合成包括放射免疫分析、生物合成标记技术、 Northern印迹分析和高效液相/电化学检测技术。这个 神经元传入对SCG细胞的影响，即秒刺激 信使系统，钙流量，去极化，和特定的受体- 对SCG NPY生产的中介刺激将被检验和关联 到加州的生产水平。影响SCG神经递质变化的因素 NPY从儿茶酚胺能到胆碱能表型的表达 我们将对表情进行检查。在共文化研究中，SCG的作用 神经递质/神经肽表达的靶细胞将是 下定决心。这些研究将在以下方面产生重大影响 建立复杂和整合的信号如何引导正常神经元 发育和生理功能。