SELECTIVE AUTOPHAGIC DEGRADATION OF PEROXISOMES IN YEAST

描述：拟议的研究涉及研究分子 控制过氧化物酶体选择性降解的机制 毕赤酵母建议分离出有缺陷的突变体 在甲醇降解过氧化物酶体的降解中， 这些生物化学和细胞学上的。过氧化物酶体降解基因 将通过功能互补分离，测序和氨基酸 酸序列推断。将比较突变体和野生型序列 以提供关于功能的见解。拟议的第二个目标是 研究是通过分离过氧化物酶体生物合成缺陷突变体， 发展积极选择的新方法。生理 将研究这些突变体的特性。

DESCRIPTION: The proposed research involves investigating the molecular mechanisms which control the selective degradation of peroxisomes in Pichia pastoris. It is proposed to isolate mutants that are defective in the degradation of methanol degrading peroxisomes and to characterize these biochemically and cytologically. The peroxisome degrading genes will be isolated by functional complementation, sequenced and the amino acid sequence deduced. Mutant and wild type sequences will be compared to provide insight concerning function. The second aim of the proposed research is to isolate peroxisome biogenesis defective mutants via the development of new approaches for positive selection. The physiological characteristics of these mutants will be studied.