SELECTIVE AUTOPHAGIC DEGRADATION OF PEROXISOMES IN YEAST

酵母中过氧化物酶体的选择性自噬降解

• 批准号: 2292219
• 负责人: James Michael Cregg
• 金额: $ 2万
• 依托单位: OREGON GRADUATE INSTITUTE SCIENCE & TECH
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-30 至 1998-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2292219
• 关键词: SELECTIVE; AUTOPHAGIC; DEGRADATION; PEROXISOMES; YEAST

DESCRIPTION: The proposed research involves investigating the molecular mechanisms which control the selective degradation of peroxisomes in Pichia pastoris. It is proposed to isolate mutants that are defective in the degradation of methanol degrading peroxisomes and to characterize these biochemically and cytologically. The peroxisome degrading genes will be isolated by functional complementation, sequenced and the amino acid sequence deduced. Mutant and wild type sequences will be compared to provide insight concerning function. The second aim of the proposed research is to isolate peroxisome biogenesis defective mutants via the development of new approaches for positive selection. The physiological characteristics of these mutants will be studied.

描述：拟议的研究包括研究分子