SELECTIVE AUTOPHAGIC DEGRADATION OF PEROXISOMES IN YEAST

酵母中过氧化物酶体的选择性自噬降解

• 批准号: 2546693
• 负责人: James Michael Cregg
• 金额: $ 2万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-30 至 1998-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2546693
• 关键词: cooperative study; electron microscopy; gene complementation; genetic library; lysosomes; molecular cloning; mutant; peroxisome; protein sequence; vesicle /vacuole; yeasts

DESCRIPTION: The proposed research involves investigating the molecular mechanisms which control the selective degradation of peroxisomes in Pichia pastoris. It is proposed to isolate mutants that are defective in the degradation of methanol degrading peroxisomes and to characterize these biochemically and cytologically. The peroxisome degrading genes will be isolated by functional complementation, sequenced and the amino acid sequence deduced. Mutant and wild type sequences will be compared to provide insight concerning function. The second aim of the proposed research is to isolate peroxisome biogenesis defective mutants via the development of new approaches for positive selection. The physiological characteristics of these mutants will be studied.

描述：拟议的研究涉及研究分子 控制过氧化物酶体选择性降解的机制 毕赤酵母建议分离出有缺陷的突变体 在甲醇降解过氧化物酶体的降解中， 这些生物化学和细胞学上的。过氧化物酶体降解基因 将通过功能互补分离，测序和氨基酸 酸序列推断。将比较突变体和野生型序列 以提供关于功能的见解。拟议的第二个目标是 研究是通过过氧化物酶体生物合成缺陷突变体来分离 发展积极选择的新方法。生理 将研究这些突变体的特性。