ASTROCYTE-EXPRESSED GENES INFLUENCING NEURITE TARGETING

影响神经突靶向的星形胶质细胞表达基因

• 批准号: 2293208
• 负责人: RAFAL M SMIGRODZKI
• 金额: $ 3.17万
• 依托单位: UNIV OF MED/DENT NJ-R W JOHNSON MED SCH
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-30 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2293208
• 关键词: ASTROCYTE; EXPRESSED; GENES; INFLUENCING; NEURITE

The proposed research should contribute to the understanding of axonal targeting in the nervous system (NS). Knowledge about this process could help in designing of somatic gene therapy in he NS, especially in some degenerative disorders and in mechanical damage to the NS. Molecular biology techniques (cDNA cloning. mRNA fingerprinting, sequencing, and sequence analysis) are to be used in conjunction with astrocyte culturing to find genes regionally expressed in population of astrocytes. Culture, purified astrocytes from various parts of neonatal rat brain will be used as a source of mRNA which will be analyzed by mRNA fingerprinting to find the differentially expressed cDNA clones in fetal rat brain cDNA libraries. The selected cDNA's will be sequenced and the sequences will be searched for clues about their function. If time allows it will be attempted to verify the functional importance of the differentially expressed cDNA's in anti-sense mRNA experiments with co-cultures of astrocytes and neurons.

这项研究将有助于对轴突的理解。 在神经系统（NS）中定位。 了解这个过程可以 帮助设计体细胞基因治疗在他NS，特别是在一些 退行性疾病和NS的机械损伤。 分子生物学技术（cDNA克隆。mRNA指纹图谱， 测序和序列分析）将与 星形胶质细胞培养，以寻找在群体中区域表达的基因， 星形胶质细胞 新生大鼠脑不同部位星形胶质细胞的纯化培养 将用作mRNA的来源，其将通过mRNA分析 指纹分析法寻找胎儿差异表达的cDNA克隆 大鼠脑cDNA文库。 将对选定的cDNA进行测序， 将搜索序列以寻找关于其功能的线索。 如果时间允许，将尝试验证功能重要性 反义mRNA实验中差异表达的cDNA 星形胶质细胞和神经元的共培养物。