CELL SURFACE GLYCOPROTEINS AND PLACE DEPENDENT SUPPRESSION OF CELL PROLIFERATION

细胞表面糖蛋白和位置依赖性细胞增殖抑制

• 批准号: 3734607
• 负责人: DOMINICK BASILE
• 金额: --
• 依托单位: HERBERT H. LEHMAN COLLEGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3734607
• 关键词: Compositae; affinity chromatography; cell cell interaction; cell cycle; cell growth regulation; cell membrane; extracellular matrix; gas chromatography; glycoprotein structure; glycoproteins; glycosylation; high performance liquid chromatography; hydroxyproline; inhibitor /antagonist; plant growth regulators; protein structure function; western blottings

Arabinogalactan proteins (AGPs) comprise a large family of hydroxyproline- containing glycoproteins associated with plant cell surfaces, both the plasmalemma and the extra-cellular matrix. Evidence for their having important roles in plant growth and development is beginning to accumulate at an accelerating rate. Among other things, they have been implicated as determinants of identity and as morphoregulatory molecules. We have obtained experimental evidence that a morphoregulatory role for certain AGPs may derive from their capacity to bring about and/or maintain potentially proliferating cells in a state of suppression. AGPs exhibit a range in buoyant densities from 1.55-1.73 in the plants we are working with. We found that surface-bound AGP fractions with buoyant densities about l.62 in Gymnocolea inflata gametophytes and l.70 in Lactuca sativa v Romana stem pith in which cell proliferation is normally suppressed, are absent from plants/tissues in which cell proliferation is experimentally "desuppressed". We characterize the AGP fractions that become "released" as HBD- (high buoyant density) AGPs and those that remain bound as LBD- (low buoyant density) AGPs. The different density classes are readily separated in total AGP extracts using CsCl gradient analysis. We propose to separate and compare the chemical compositions of the HBD- with LBD- AGPs of G. inflata as well as of L. sativa to see if there is something other than the degree of glycosylation that distinguishes the density classes. We will also compare HBD-AGPs of G. inflata with those of L. sativa to see if we can find some common, conserved feature(s) in this class in which binding properties change correlated with suppressed vs. desuppressed cell proliferation. The combined, parallel comparative chemical analysis of the liverwort gametophytes and the lettuce stem tissue are expected to provide a clue as to how the presence of certain HBD-AGPs at the cell surface helps maintain potentially proliferating cells in a suppressed state.

阿拉伯半乳聚糖蛋白（AGPs）包括一个大的羟脯氨酸-半乳聚糖蛋白家族。 含有与植物细胞表面相关的糖蛋白， 质膜和细胞外基质 证明他们拥有 在植物生长和发育中的重要作用开始积累 以加速的速度。 除其他外，他们被牵连， 身份的决定因素和作为形态调节分子。 我们有 获得的实验证据表明，某些形态调节作用 AGPs可能源于其产生和/或维持 潜在的增殖细胞处于抑制状态。 AGPs展示 在我们正在研究的工厂中，浮力密度的范围从1.55-1.73 以. 我们发现具有浮力密度的表面结合AGP组分 裸鞘花配子体为1.62，莴苣为1.70 v细胞增殖通常受到抑制的Romana茎髓， 不存在于细胞增殖实验性地 “解除压抑” 我们描述了“释放”的AGP组分， 作为HBD-（高浮力密度）AGPs和那些保持结合作为LBD- (low浮力密度）AGPs。 不同的密度等级很容易 使用CsCl梯度分析在总AGP提取物中分离。 我们提出 分离并比较HBD与LBD的化学组成， G. inflata和L.看看有没有什么 除了糖基化程度， 班 我们还将比较G. inflata与L. 看看我们是否能找到一些共同的，保守的特征（S）， 类，其中绑定属性的更改与抑制与 去抑制细胞增殖。 组合的、平行的比较 苔类配子体和莴苣茎的化学分析 组织有望提供线索， 细胞表面的HBD-AGPs有助于维持潜在的增殖 细胞处于抑制状态。