HYDROXYPROLINE-RICH PROTEINS IN PLAGIOCHILA ARTICA LEAF DEVELOPMENT REGULATION

北冰洋叶发育调控中富含羟基脯氨酸的蛋白质

• 批准号: 3937189
• 负责人: DOMINICK BASILE
• 金额: --
• 依托单位: HERBERT H. LEHMAN COLLEGE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3937189
• 关键词: cell growth regulation; gene expression; histochemistry /cytochemistry; histogenesis; hydroxylation; hydroxyproline; immunochemistry; leaf; plant growth regulators; protein structure

Separate experiments in which antagonists of prolyl-hydroxylase and each of its cosubstrates/cofactors were supplied to axenic cultures of developing plants of Plagiochila arctica (Hepaticae) had the same result: leaf primordia that are normally suppressed at a very early stage of development continued to develop into full-sized leaves. Since the function of the prolyl-hydroxylase system is the posttranslational conversion of petidyl-proline to peptidyl-hydroxyproline, we tentatively conclude that prolyl- hydroxylase inhibitor-induced desuppression is related to altered synthesis of hydroxyproline-containing proteins (Hyp-proteins). There are two classes of Hyp-proteins widely distributed in green land plants, each with more than one "species" of protein. Both classes are represented in P. arctica, but the number of representatives of each class is not known yet. We propose here: 1. to find out how many Hyp-proteins of each class are synthesized in P. arctica; 2. to more directly test the assumption that the prolyl-hydroxylase inhibitors we used are in fact limiting posttranslational hydroxylation of proline in P. arctica; and 3. to use immunohistochemical procedures to correlate the presence/absence of specific Hyp-proteins identified in P. artica with suppression/desuppression of leaf development. These investigations of suppression/desuppression of cell development bear on several basic biological questions. First, what factors control cell multiplication and cell shape and secondly, what evolutionary role these suppressive effects play in altering phenotypic development? In short, we are seeing the biochemistry whereby phenotype develops from genotype. When suppression occurs during the course of histogenesis, organogenesis or ontogeny largely determines size. Where suppression occurs relative to other developing parts, it largely determines shape. Whether suppression occurs or nor largely determines whether growth and development will be "determinate" vs "indeterminate", or "normal" vs "abnormal" (pathological).

在单独的实验中，Pro-羟基酶的拮抗剂 并且它的每一种辅基/辅因子被提供给无菌 北极斜纹夜蛾发育植株的培养 得到了同样的结果：通常被抑制的叶原基 在非常早期的发展阶段，继续发展为 全尺寸的树叶。由于Pro-羟基酶的功能 系统是酰基-脯氨酸的翻译后转化为 肽基-羟基脯氨酸，我们初步得出结论： 羟基酶抑制剂诱导的降压与改变有关 含羟脯氨酸蛋白(Hyp-Proteins)的合成。 绿色植物中广泛分布的Hyp蛋白有两类 陆地植物，每种植物都有不止一种蛋白质。两者都有 类用P.arctica表示，但 目前还不清楚每个班级的代表。我们在此建议： 1.计算每类超蛋白的合成数量。 2.更直接地检验这一假设 我们使用的Pro-羟基酶抑制剂实际上限制了 北极星天牛体内脯氨酸的翻译后羟化作用； 使用免疫组织化学程序来关联 青海对虾中特异性Hyp蛋白的存在/缺失 对叶片发育具有抑制/去抑制作用。 这些关于细胞抑制/去抑制的研究 发展涉及几个基本的生物学问题。第一, 控制细胞增殖和细胞形态的因素是什么？ 其次，这些抑制效应在进化中起到了什么作用？ 改变表型发育？简而言之，我们正在看到 表型由基因发展而来的生物化学。 当在组织发生过程中发生抑制时， 器官发生或个体发育在很大程度上决定了大小。哪里 抑制发生在相对于其他发展中的部分，它在很大程度上 决定形状。无论镇压是否发生在很大程度上 决定增长和发展是否将是“确定的”与 “不确定”，或“正常”对“异常”(病态)。