HOMEOBOX GENES AND THE MOLECULAR CONTROL OF LIMB PATTERN

描述：这项提案代表了 HOX基因在肢体发育过程中的表达塔宾认为 特定HOX基因的表达控制着阶段和区域的特异性 肢芽中的细胞增殖。塔宾实验室之前的工作 已经表明这些基因是以嵌套的模式沿着 肢芽的近端、远端和前后轴。在预赛中 实验中，Tabin描述了一个几乎 雏鸡体内这些基因的全套。在当前应用程序中，Tabin 建议比较错误表达个体的表型后果 并改变错误表达的次数，以确定是否 这些基因影响未分化细胞团的增殖或生长。 已建立的骨骼元素。将对表型结果进行分析 从形态和细胞增殖的角度来鉴定 受影响的特定细胞。在第二个项目中，塔宾将使用差分 显示以识别HOX基因表达的下游靶点。这些 靶点将被用来检查HOX基因对TERS的特异性 特定的目标。

DESCRIPTION: This proposal represents a global characterization of the expression of HOX genes during the development of the limb. Tabin argues that expression of specific hox genes controls stage and region specific cell proliferation in the limb bud. Previous work from the Tabin laboratory has shown that these genes are expressed in a nested patterns along the proximal distal and anterior posterior axis of the limb bud. In preliminary experiments, Tabin describes the isolation and expression of an almost complete set of these genes in chick. In the current application, Tabin proposes to compare the phenotypic consequences of misexpressing individual HOX in the limb and to alter the times of misexpression to determine whether these genes affect proliferation of the undifferentiated cell mass or growth of established skeletal elements. Phenotypic consequences will be assayed both morphologically and in terms of cell proliferation to identify the specific cells affected. In a second project Tabin will use differential display to identify downstream targets of HOX gene expression. These targets will be used to examine the specificity of HOX genes for there specific targets.