DISCOVERY AND EXPRESSION OF NEW ANTICHOLINERGIC TOXINS

新抗胆碱能毒素的发现和表达

• 批准号: 2517013
• 负责人: Lincoln T. Potter
• 金额: $ 19.55万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-09-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2517013
• 关键词: anticholinergic agent; complementary DNA; molecular cloning; muscarinic receptor; neurotoxins; protein folding; receptor binding; recombinant DNA; recombinant proteins; reptile poison; snakes

DESCRIPTION: (adapted from Applicant's Abstract) The investigators have shown that the venom of the Eastern green mamba from Africa, Dendroaspis angusticeps, is an excellent source for toxins that distinguish the m1 and m4 subtypes of muscarinic receptors, and have preliminary evidence for the presence of toxins specific for m2 receptors. The anti-muscarinic and toxins are present only in trace amounts. They therefore hypothesize that the toxins of most interest can be best identified by a combination of protein purification and recombinant DNA approaches, and obtained most easily in useful quantities by expression of the corresponding cDNA clones.

描述：（改编自申请人摘要）研究者已 表明来自非洲的东部绿色曼巴树盾蛇的毒液 窄头绦虫，是一个很好的来源，毒素，区分m1 和m4亚型的毒蕈碱受体，并有初步证据表明， M2受体特异性毒素的存在抗毒蕈碱药 而毒素只有微量 因此他们假设 最令人感兴趣的毒素可以通过组合 蛋白质纯化和重组DNA方法，并获得了大多数 通过表达相应的cDNA， 克隆

项目成果

Site-directed mutagenesis of m1-toxin1: two amino acids responsible for stable toxin binding to M(1) muscarinic receptors.

• 发表时间: 2001-10
• 期刊: Molecular pharmacology
• 影响因子: 3.6
• 作者: J. Krajewski;I. Dickerson;L. Potter