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DISCOVERY AND EXPRESSION OF NEW ANTICHOLINERGIC TOXINS

DISCOVERY AND EXPRESSION OF NEW ANTICHOLINERGIC TOXINS
新抗胆碱能毒素的发现和表达
批准号：
2517013
负责人：
Lincoln T. Potter
金额：
$19.55万
依托单位：
UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
1995
资助国家：
美国
项目状态：
已结题
起止时间：
1995-09-01 至 1999-08-31
来源：
https://reporter.nih.gov/project-details/2517013
关键词：
anticholinergic agent complementary DNA molecular cloning muscarinic receptor neurotoxins protein folding receptor binding recombinant DNA recombinant proteins reptile poison snakes

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中文摘要
翻译
描述：(改编自申请者摘要)调查人员已 显示了来自非洲的东部绿色曼巴的毒液，Dendroaspis 是区分M1的毒素的一个很好的来源 和M4亚型的M受体，并有初步证据 对于M2受体特异性毒素的存在。反毒枭 而且毒素只以微量存在。因此，他们假设 最感兴趣的毒素可以通过组合来最好地识别 蛋白质纯化和重组DNA的方法，获得了最多 通过相应的cDNA的表达而容易地获得有用的数量 克隆人。
英文摘要
DESCRIPTION: (adapted from Applicant's Abstract) The investigators have shown that the venom of the Eastern green mamba from Africa, Dendroaspis angusticeps, is an excellent source for toxins that distinguish the m1 and m4 subtypes of muscarinic receptors, and have preliminary evidence for the presence of toxins specific for m2 receptors. The anti-muscarinic and toxins are present only in trace amounts. They therefore hypothesize that the toxins of most interest can be best identified by a combination of protein purification and recombinant DNA approaches, and obtained most easily in useful quantities by expression of the corresponding cDNA clones.
期刊论文(5)
专著(0)
科研奖励(0)
会议论文
Site-directed mutagenesis of m1-toxin1: two amino acids responsible for stable toxin binding to M(1) muscarinic receptors.
DOI： --
发表时间： 2001-10
期刊： Molecular pharmacology
影响因子： 3.6
作者： [J. Krajewski;I. Dickerson;L. Potter]
通讯作者： J. Krajewski;I. Dickerson;L. Potter
DISCOVERY AND EXPRESSION OF NEW ANTICHOLINERGIC TOXINS
DISCOVERY AND EXPRESSION OF NEW ANTICHOLINERGIC TOXINS
CHOLINERGIC REPLACEMENT THERAPY
CHOLINERGIC REPLACEMENT THERAPY
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