MOLECULAR PATHOGENESIS OF H MUTLELAE GASTRITIS

混血儿胃炎的分子发病机制

• 批准号: 2517140
• 负责人: KARL A ANDRUTIS
• 金额: $ 4.07万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-30 至 1997-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2517140
• 关键词: Helicobacter; bacterial antigens; bacterial cytopathogenic effect; bacterial genetics; bacterial proteins; bactericidal immunity; disease /disorder model; ferrets; gastritis; genetic strain; histopathology; molecular cloning; molecular pathology; mutant; serology /serodiagnosis; urease; virulence; western blottings

DESCRIPTION: H. pylori is a major cause of gastrointestinal disease worldwide. Recently, several putative virulence factors of Helicobacter organisms have been identified. Characterization of these determinants and investigation into their mechanisms of action is an important area of study in Helicobacter pathogenesis. Molecular biological techniques have allowed research in this field to progress rapidly in both the identification and characterization of virulence factors and in the ability to study these determinants in vivo. The H. mustelae ferret model has been thoroughly characterized and is the only naturally-infected animal model to develop ulcer disease. Several isogenic mutant strains of H. mustelae lacking individual virulence factors have been constructed and offer a unique opportunity to investigate the mechanisms of Helicobacter pathogenesis. The objectives of this research are to characterize the virulence factors of H. mustelae and to conduct in vivo infection studies using isogenic mutant strains of H. mustelae in the ferret model. Characterization of the effects of individual virulence determinants will increase our understanding of Helicobacter pathogenesis and assist in therapeutic and preventative measures.

描述：幽门螺杆菌是引起胃肠道疾病的主要原因。 全世界。近年来，幽门螺杆菌的几个可能的毒力因子 生物体已经被鉴定出来。这些决定因素的特征和 研究它们的作用机制是一个重要的研究领域。 在幽门螺杆菌的发病机制中。分子生物学技术已经使 这一领域的研究将在识别和识别方面取得快速进展 毒力因子的特征和研究这些因子的能力 体内的决定因素。芥子雪貂的模型已经被彻底 是唯一一种自然感染的动物模型 溃疡病。几个缺乏芥子虫同基因突变株 个体毒力因子已经被构建并提供了一个独特的 有机会研究幽门螺杆菌的致病机制。这个 本研究的目的是对H。 利用同基因突变体进行体内感染研究 雪貂模型中的芥子虫菌株。对影响的表征 单个毒力决定因素的研究将增加我们对 幽门螺杆菌的致病机制及辅助治疗和预防 措施。