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MOLECULAR PATHOGENESIS OF H MUTLELAE GASTRITIS

混血儿胃炎的分子发病机制

基本信息

批准号：
2694886
负责人：
KARL A ANDRUTIS
金额：
$ 10.08万
依托单位：
TUFTS UNIVERSITY BOSTON
依托单位国家：
美国
项目类别：
财政年份：
1996
资助国家：
美国
起止时间：
1996-09-30 至 2001-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2694886
关键词：
Helicobacter bacterial antigens bacterial cytopathogenic effect bacterial genetics bacterial proteins bactericidal immunity disease /disorder model ferrets gastritis genetic strain histopathology molecular cloning molecular pathology mutant serology /serodiagnosis urease virulence western blottings

项目摘要

DESCRIPTION: H. pylori is a major cause of gastrointestinal disease worldwide. Recently, several putative virulence factors of Helicobacter organisms have been identified. Characterization of these determinants and investigation into their mechanisms of action is an important area of study in Helicobacter pathogenesis. Molecular biological techniques have allowed research in this field to progress rapidly in both the identification and characterization of virulence factors and in the ability to study these determinants in vivo. The H. mustelae ferret model has been thoroughly characterized and is the only naturally-infected animal model to develop ulcer disease. Several isogenic mutant strains of H. mustelae lacking individual virulence factors have been constructed and offer a unique opportunity to investigate the mechanisms of Helicobacter pathogenesis. The objectives of this research are to characterize the virulence factors of H. mustelae and to conduct in vivo infection studies using isogenic mutant strains of H. mustelae in the ferret model. Characterization of the effects of individual virulence determinants will increase our understanding of Helicobacter pathogenesis and assist in therapeutic and preventative measures.

描述：幽门螺杆菌是胃肠道疾病的主要原因全世界。最近，一些推测的螺杆菌毒力因子生物体已被鉴定。这些决定因素的表征和研究其作用机制是一个重要的研究领域在螺杆菌发病机制中。分子生物学技术已允许该领域的研究在识别和鉴定方面都取得了迅速进展毒力因子的特征以及研究这些因子的能力体内的决定因素。 H. Mustelae 雪貂模型已被彻底已被表征，并且是唯一开发的自然感染动物模型溃疡病。几种鼬鼠等基因突变株缺乏已构建了个体毒力因子并提供了独特的研究螺杆菌发病机制的机会。这本研究的目的是表征幽门螺杆菌的毒力因子。鼬并使用同基因突变体进行体内感染研究雪貂模型中的鼬鼠菌株。效果表征个体毒力决定因素的研究将增加我们对螺杆菌发病机制及其辅助治疗和预防措施。