INVESTIGATION INTO THE PATHOGENESIS OF CHRONIC SINUSITIS

慢性鼻窦炎发病机制的探讨

• 批准号: 2442374
• 负责人: Lisa M Wheatley
• 金额: $ 8.69万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-15 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2442374
• 关键词: B lymphocyte; antibody formation; chronic disease /disorder; clinical research; helper T lymphocyte; human subject; immunoglobulin G; in situ hybridization; interferon gamma; interleukin 13; interleukin 4; interleukin 5; pathologic process; polymerase chain reaction; protein biosynthesis; serology /serodiagnosis; sinusitis; tissue /cell culture

DESCRIPTION: a. Candidate: Dr. Lisa Wheatley received her M.D. from Loyola University in Chicago in 1986 and trained in internal medicine at the University of Iowa. Following that, she served as a volunteer in India for six months and then trained in allergy and clinical immunology at the University of Pennsylvania, where she was a fellow. In 1994 she was appointed assistant professor in the Department of Internal Medicine at the University of Virginia. Her goal is to develop a new and independent research program at that institution. b. Sponsor: Dr. Thomas Platts-Mills is Professor of Medicine and Head, Division of Allergy and Clinical Immunology at the University of Virginia. c. Environment: The research will be carried out in the Division of Allergy and Clinical immunology at the University of Virginia Health Sciences Center. d. Training Plan: The project is based on the hypothesis that chronic sinusitis is an inflammatory and perhaps immunological disease. Chronic sinusitis was estimated in 1986 to be the most frequent chronic disease in the U.S., affecting over 30 million individuals. Dr. Wheatley proposes to investigate the role of IgG4 in chronic sinusitis by: (1) detecting, in serum, IgG4 that is specific for bacterial antigens commonly found in inflamed sinuses, (2) comparing the ratios of IgG4 and IgG1 to albumin in nasal washings and serum, (3) using RT-PCR to localize IgG4 mRNA in sinus tissue, and (4) using anti-CD40, coculture, and cytokines to examine B cell production of IgG4 without IgE. Dr. Wheatley will also investigate the role of Th2 responses in chronic sinusitis by measuring cytokine expression by bulk culture and cloned T cells isolated from inflamed sinuses and then comparing these responses to those for Th1 antigen and allergen.

产品说明： a.候选人：丽莎惠特利博士获得了医学博士学位。来自洛约拉大学， 1986年在芝加哥，并在爱荷华州大学接受内科学培训。 之后，她在印度做了六个月的志愿者， 他曾在芝加哥大学接受过过敏和临床免疫学方面的培训， 宾夕法尼亚州，她是那里的一个研究员。 1994年，她被任命为助理 他是纽约大学内科学系的教授， 弗吉尼亚 她的目标是开发一个新的和独立的研究计划， 这个机构。 B.赞助商：托马斯普拉茨-米尔斯博士是医学教授和负责人， 弗吉尼亚大学变态反应和临床免疫学系。 C.环境：研究将在过敏症部门进行 弗吉尼亚健康科学大学的临床免疫学 中心 D.培训计划：该项目是基于假设，慢性 鼻窦炎是一种炎症性疾病，也可能是免疫性疾病。 慢性 1986年，鼻窦炎被估计为美国最常见的慢性疾病。 美国，影响超过三千万人 惠特利博士提议 探讨IgG 4在慢性鼻窦炎中的作用：（1）检测， 血清，IgG 4，对常见于 炎症鼻窦，（2）比较IgG 4和IgG 1与白蛋白的比例， （3）采用RT-PCR法检测IgG 4 mRNA在鼻窦组织中的定位 （4）用抗CD 40抗体、共培养和细胞因子检测B细胞 不含IgE的IgG 4的产生。 惠特利博士还将研究 慢性鼻窦炎中Th 2应答的研究 从发炎的鼻窦中分离大量培养和克隆的T细胞， 将这些反应与Th 1抗原和过敏原的反应进行比较。