POSTTRANSCRIPTIONAL REGULATION OF C-MYC IN C2 MYOBLASTS

C2 成肌细胞中 C-MYC 的转录后调控

• 批准号: 2442759
• 负责人: NEWMAN M YEILDING
• 金额: $ 8.98万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-07-10 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2442759
• 关键词: RNA biosynthesis; cell differentiation; fusion gene; gene expression; genetic regulatory element; messenger RNA; myoblasts; nucleic acid sequence; polymerase chain reaction; posttranscriptional RNA processing; protooncogene; tissue /cell culture; transcription factor

The c-myc proto-oncogene encodes a nuclear phosphoprotein which normally functions as a transcription regulatory factor and is thought to play an important role in cell proliferation and differentiation. Deregulated expression of c-myc has been associated with neoplastic transformation. Defining the regulatory mechanisms affecting c-myc expression may help to better understand the molecular mechanisms of carcinogenesis. Post- transcriptional gene regulation has been shown to be an important determinant of c-myc expression. The proposed studies are designed to define the cis-acting elements determining steady-state expression of c- myc and mediating down-regulation of c-myc expression during cellular differentiation and to define the mechanisms of regulation. Based on results of these studies, we will begin to characterize and identify the trans-regulatory factors mediating c-myc regulation. Common regulatory motifs will be sought between c-myc and other short-lived mRNAs, including those encoding other factors involved in cell proliferation and differentiation.

c-myc 原癌基因编码一种核磷蛋白，通常 作为转录调节因子，被认为发挥着 在细胞增殖和分化中发挥重要作用。 放松管制 c-myc 的表达与肿瘤转化有关。 定义影响 c-myc 表达的调控机制可能有助于 更好地了解致癌的分子机制。 邮政- 转录基因调控已被证明是一个重要的 c-myc 表达的决定因素。 拟议的研究旨在 定义决定 c-稳态表达的顺式作用元件 myc 并介导细胞过程中 c-myc 表达的下调 差异化并定义调节机制。 基于 根据这些研究的结果，我们将开始描述和识别 介导 c-myc 调节的反式调节因子。 共同监管 将在 c-myc 和其他短寿命 mRNA 之间寻找基序，包括 那些编码参与细胞增殖的其他因子和 差异化。