POSTTRANSCRIPTIONAL REGULATION OF C-MYC IN C2 MYOBLASTS

C2 成肌细胞中 C-MYC 的转录后调控

• 批准号: 6029897
• 负责人: NEWMAN M YEILDING
• 金额: $ 11.19万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-07-10 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6029897
• 关键词: RNA biosynthesis; cell differentiation; fusion gene; gene expression; genetic regulatory element; messenger RNA; myoblasts; nucleic acid sequence; polymerase chain reaction; posttranscriptional RNA processing; protooncogene; tissue /cell culture; transcription factor

The c-myc proto-oncogene encodes a nuclear phosphoprotein which normally functions as a transcription regulatory factor and is thought to play an important role in cell proliferation and differentiation. Deregulated expression of c-myc has been associated with neoplastic transformation. Defining the regulatory mechanisms affecting c-myc expression may help to better understand the molecular mechanisms of carcinogenesis. Post- transcriptional gene regulation has been shown to be an important determinant of c-myc expression. The proposed studies are designed to define the cis-acting elements determining steady-state expression of c- myc and mediating down-regulation of c-myc expression during cellular differentiation and to define the mechanisms of regulation. Based on results of these studies, we will begin to characterize and identify the trans-regulatory factors mediating c-myc regulation. Common regulatory motifs will be sought between c-myc and other short-lived mRNAs, including those encoding other factors involved in cell proliferation and differentiation.

C-myc原癌基因编码一种核磷蛋白，它通常 作为转录调节因子发挥作用，被认为是一种 在细胞增殖和分化中发挥重要作用。放松管制 C-myc的表达与肿瘤转化有关。 明确影响c-myc表达的调控机制可能有助于 更好地理解致癌的分子机制。邮寄- 转录基因调控已被证明是一种重要的 C-myc表达的决定因素。拟议的研究旨在 定义决定c-的稳态表达的顺式作用元件 MYC与细胞内c-myc表达下调 并对调控机制进行界定。基于 这些研究的结果，我们将开始表征和确定 介导c-myc调控的跨调控因子。共同监管 将在c-myc和其他短暂的mRNAs之间寻找基序，包括 这些编码其他与细胞增殖有关的因子和 差异化。

项目成果

Coding elements in exons 2 and 3 target c-myc mRNA downregulation during myogenic differentiation.

外显子 2 和 3 中的编码元件针对肌原性分化过程中 c-myc mRNA 的下调。

• DOI: 10.1128/mcb.17.5.2698
• 发表时间: 1997
• 期刊: Molecular and cellular biology
• 影响因子: 5.3
• 作者: Yeilding,NM;Lee,WM
• 通讯作者: Lee,WM

Gallic Acid-Laminarin Conjugate Is a Better Antioxidant than Sulfated or Carboxylated Laminarin.

• DOI: 10.3390/antiox9121192
• 发表时间: 2020-11-27
• 期刊: Antioxidants (Basel, Switzerland)
• 作者: Fernandes-Negreiros MM;Batista LANC;Silva Viana RL;Araujo Sabry D;Paiva AAO;Paiva WS;Machado RIA;Sousa Junior FL;de Lima Pontes D;Vitoriano JO;Alves Junior C;Lanzi Sassaki G;Rocha HAO
• 通讯作者: Rocha HAO

Identification of sequences in c-myc mRNA that regulate its steady-state levels.

鉴定 c-myc mRNA 中调节其稳态水平的序列。

• DOI: 10.1128/mcb.16.7.3511
• 发表时间: 1996
• 期刊: Molecular and cellular biology
• 影响因子: 5.3
• 作者: Yeilding,NM;Rehman,MT;Lee,WM
• 通讯作者: Lee,WM

Gallic Acid-Dextran Conjugate: Green Synthesis of a Novel Antioxidant Molecule.

没食子酸-葡聚糖缀合物：新型抗氧化剂分子的绿色合成。

• DOI: 10.3390/antiox8100478
• 发表时间: 2019
• 期刊: Antioxidants (Basel, Switzerland)
• 作者: Queiroz,MoacirFernandes;Sabry,DiegoAraujo;Sassaki,GuilhermeLanzi;Rocha,HugoAlexandreOliveira;Costa,LeandroSilva
• 通讯作者: Costa,LeandroSilva