POSTTRANSCRIPTIONAL REGULATION OF C-MYC IN C2 MYOBLASTS

c-myc原癌基因编码一种核磷蛋白， 作为转录调节因子发挥作用， 在细胞增殖和分化中起重要作用。 放松管制 c-myc的表达与肿瘤转化有关。 明确影响c-myc表达的调控机制可能有助于 更好地了解致癌的分子机制。 后 转录基因调控已被证明是一个重要的 c-myc表达的决定因素。 拟议的研究旨在 定义决定c-的稳态表达的顺式作用元件 myc和介导c-myc表达下调 差异化和确定监管机制。 基于 根据这些研究的结果，我们将开始表征和识别 介导c-myc调节的反式调节因子。 共同的监管 将在c-myc和其他短寿命mRNA之间寻找基序，包括 那些编码参与细胞增殖的其他因子的基因， 分化

The c-myc proto-oncogene encodes a nuclear phosphoprotein which normally functions as a transcription regulatory factor and is thought to play an important role in cell proliferation and differentiation. Deregulated expression of c-myc has been associated with neoplastic transformation. Defining the regulatory mechanisms affecting c-myc expression may help to better understand the molecular mechanisms of carcinogenesis. Post- transcriptional gene regulation has been shown to be an important determinant of c-myc expression. The proposed studies are designed to define the cis-acting elements determining steady-state expression of c- myc and mediating down-regulation of c-myc expression during cellular differentiation and to define the mechanisms of regulation. Based on results of these studies, we will begin to characterize and identify the trans-regulatory factors mediating c-myc regulation. Common regulatory motifs will be sought between c-myc and other short-lived mRNAs, including those encoding other factors involved in cell proliferation and differentiation.