PREDICTING PROGRESSION TO DYSPLASIA--ULCERATIVE COLITIS

预测进展为异型增生——溃疡性结肠炎

• 批准号: 2414344
• 负责人: Teresa A Brentnall
• 金额: $ 7.87万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-05-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2414344
• 关键词: biopsy; blood tests; cancer risk; carcinogenesis; colon neoplasms; colon surgery; diagnosis design /evaluation; endoscopy; flow cytometry; gastrointestinal imaging /visualization; gene mutation; genetic mapping; genetic markers; human subject; loss of heterozygosity; neoplasm /cancer diagnosis; neoplasm /cancer genetics; polymerase chain reaction; restriction fragment length polymorphism; tumor suppressor genes; ulcerative colitis

I have almost completed my fellowship in gastroenterology and am using my new skills as a clinical investigator and molecule geneticist to solve problems in early diagnosis an pathogenesis of gastrointestinal cancer. i worked for six years in molecular biology medical school and have partially caught up with progress in medical genetics which occurred during medical school and my residency. I have been given the opportunity under supervision to independently pose hypotheses, to design experiments and to carry them out. I have found that working with ulcerative colitis patients at high risk for developing cancer is an excellent way to further my primary interest in reducing to clinical practice the discoveries of basic science. I have a strong desire to learn more about the pathogenesis of gastrointestinal cancer. The type of research done in this laboratory and the colleagues available to advise me are ideal ways to attain this goal. My primary sponsor is Dr. Rubin clinical investigator, gastroenterologist, and past mentor of many excellent academic gastroenterologists. My co- sponsors are Dr. Rodger Haggitt, an outstanding gastrointestinal pathologist and Dr. Sum Lee, a superb clinical investigator teacher. The laboratory facilities available to me are more than adequate and all of my sponsors have promised to help support my research despite the current difficult financial environment. The long term objective of our research group is to gain better understanding of the molecular mechanisms of neoplastic progression in the dysplasia and cancer associated with ulcerative colitis. In the process we hope to develop earlier and more cost effective methods of diagnosing curable cancer and it's precursors. We hope to do these studies in three ways: 1. To use molecular genetic techniques on colectomy specimens to study the pathogenesis of colon cancer. 2. To prospectively study biopsies taken during surveillance colonoscopy to find markers predictive of dysplasia. 3. To develop a screening blood test to target the UC patients at highest risk for developing dysplasia or cancer. I am currently managing the day to day operation of a molecular genetics laboratory with the assistance of Dr. Rabinovitch. With the support of this K08 I hope to complete my training in molecular genetics and to continue collaborative research.

我几乎完成了我在胃肠病学的奖学金， 作为临床研究者和分子遗传学家的新技能， 胃肠道肿瘤的早期诊断和发病机制等问题。 我 我在分子生物学医学院工作了六年， 赶上了医学遗传学的进展， 学校和实习期 我得到了一个机会， 监督独立提出假设，设计实验， 把他们带出去。 我发现治疗溃疡性结肠炎患者 是一个很好的方式来进一步我的 主要兴趣是将基础研究的发现转化为临床实践， 科学 我有一个强烈的愿望，以了解更多的发病机制， 胃肠癌 在这个实验室里进行的研究类型， 可以向我提供建议的同事是实现这一目标的理想途径。 我的主要赞助人是鲁宾医生，临床研究员，胃肠病学家， 也是许多优秀学术胃肠病学家的导师 我的同事 赞助商是罗杰·哈吉特博士，一位杰出的胃肠道专家。 病理学家和一位出色的临床研究员老师李森博士。 的 实验室设施对我来说是绰绰有余，我所有的 赞助商已经承诺帮助支持我的研究，尽管目前 艰难的金融环境。 我们研究小组的长期目标是获得更好的 了解肿瘤进展的分子机制， 与溃疡性结肠炎相关的发育不良和癌症。 在这个过程中我们 希望开发出更早、更经济的诊断方法 可治愈的癌症及其前兆。 我们希望在三年内完成这些研究， 方式： 1. 利用分子遗传学技术对结肠切除标本进行研究， 结肠癌的发病机制。 2. 前瞻性研究监测结肠镜检查期间采集的活检标本 找到预测发育异常的标志物 3. 开发一种筛查血液检测，以最大限度地针对UC患者 发育不良或癌症的风险。 我目前正在管理一个分子遗传学的日常运作， 在Rabinovitch博士的帮助下，实验室。 的支持下 这个K08我希望完成我在分子遗传学方面的训练， 继续合作研究。