T LYMPHOCYTE RESPONSE TO VIRAL ANTIGENS
T LYMPHOCYTE RESPONSE TO VIRAL ANTIGENS
批准号:
2390324
负责人:
Thomas J Braciale
金额:
$22.72万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1991
资助国家:
美国
项目状态:
已结题
起止时间:
1991-07-01 至 1999-03-31
关键词:
MHC class II antigen antigen presentation autophagy bioassay cellular immunity cytotoxicity helper T lymphocyte high performance liquid chromatography human tissue immunoaffinity chromatography immunoprecipitation influenzavirus A intracellular transport lymphocyte proliferation lysosomes mass spectrometry microorganism hemagglutinin peptide structure protein purification protein sequence proteolysis synthetic peptide tissue /cell culture virus antigen virus protein
中文摘要
该建议旨在研究处理的结构
在感染细胞中产生的流感病毒抗原部分,
识别的人CD4+ T淋巴细胞,并表征
病毒蛋白加工和呈递的细胞内途径
to human人T淋巴细胞. 拟议的研究是我们的一个扩展,
正在进行的关于人T淋巴细胞对
病毒多肽。 我们的实验方法是首先描述
天然加工的流感血凝素和核衣壳肽
通过色谱和质谱手段结合到HLADRwII。 在
相关研究表明,病毒感染对自身谱系的影响
将评估与感染细胞中MHCII类分子结合的肽
以及病毒抗原形式,例如分离的病毒
多肽或从头表达的病毒蛋白质,在结构上,
在抗原呈递细胞中产生的天然加工肽。
将努力产生可以追踪以下物质的形成的试剂:
肽MHC复合物在感染的细胞,并评估的影响,
抗原表位外的氨基酸对病毒加工的影响
多肽和抗原肽的形成。 在相关
研究,我们将研究病毒糖蛋白靶向的途径,
溶酶体抗原处理区室和机制,
细胞溶质病毒蛋白被加工并与
人MHC II类分子。 本提案中概述的实验
将提供新的信息的结构,
病毒抗原部分识别人T淋巴细胞,
通过该方法它们在病毒感染的细胞中产生。 此类信息
将在病毒疫苗设计中具有直接重要性,
对了解感染过程中的免疫功能具有重要意义。
英文摘要
This proposal is designed to investigate the structure of the processed
influenza viral antigenic moieties generated in infected cells which are
recognized by human CD4+ T lymphocytes and to characterize the
intracellular pathways by which viral proteins are processed and presented
to human T lymphocytes. The proposed studies are an extension of our
ongoing work on the characterization of the human T lymphocyte response to
viral polypeptides. Our experimental approach is to first characterize
the naturally processed influenza hemagglutinin and nucleocapsid peptides
bound to HLA DRwll by chromatographic and mass spectroscopic means. In
related studies, the impact of virus infection on the spectrum of self
peptide bound to MHC class II molecules in infected cells will be assessed
as well as the effect of viral antigen form, e.g. isolated viral
polypeptides or de novo expressed viral protein, on the structure of the
naturally processed peptides generated in antigen presenting cells.
Efforts will be made to generate reagents which can tract the formation of
peptide MHC complexes in the infected cell and to evaluate the effect of
aminoacids outside of an antigenic epitope on the processing of the viral
polypeptides and the formation of the antigenic peptide. In related
studies, we will examine the pathways of viral glycoprotein targeting to
the lysosomal antigen processing compartment and the mechanism by which
cytosolic viral proteins are processed and presented in association with
human MHC class II molecule. The experiments outlined in this proposal
will provide new information on the structure of the naturally processed
viral antigenic moieties recognized human T lymphocytes, and the mechanism
by which they are generated in virus infected cells. Such information
will be of immediate importance in viral vaccine design and of long-term
significance in understanding immune function during infection.
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会议论文
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财政年份:2009
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依托单位:
Administration
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财政年份:2009
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Immune Regulation of Virus Clearance and Tissue Injury at Sites of Infection
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Immune Regulation of Virus Clearance and Tissue Injury at Sites of Infection
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依托单位:
CD8+ T cell trafficking to the normal lung
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依托单位:
CD8+ T cell trafficking to the normal lung
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CD8+ T cell trafficking to the normal lung
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CD8+ T cell trafficking to the normal lung
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RESPIRATORY SYNCYTIAL VIRUS AND ATOPIC PULMONARY RESPONSE
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资助金额:$19.31万
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财政年份:2000
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RESPIRATORY SYNCYTIAL VIRUS AND ATOPIC PULMONARY RESPONSE
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财政年份:1999
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RESPIRATORY SYNCYTIAL VIRUS AND ATOPIC PULMONARY RESPONSE
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财政年份:1998
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RESPIRATORY SYNCYTIAL VIRUS AND ATOPIC PULMONARY RESPONSE
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财政年份:1997
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负责人:Thomas J Braciale
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INTERDISCIPLINARY TRAINING PROGRAM IN IMMUNOLOGY
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依托单位:
海外基金