GAP JUNCTIONS IN OLFACTION

嗅觉中的间隙连接

• 批准号: 2014725
• 负责人: VINCENT E DIONNE
• 金额: $ 14.2万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-01 至 1999-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2014725
• 关键词: Urodela; chemoreceptors; dyes; electrophysiology; gap junctions; olfactions; receptor coupling; respiratory epithelium; sensory signal detection; voltage /patch clamp

This study is directed at identifying and characterizing the physiological roles of gap junctions in the olfactory epithelium. New data have shown that olfactory receptor neurons are coupled to one another and possibly to non-neuronal cells in the olfactory epithelium of Necturus maculosus. Coupling was revealed by dye-transfer, by electrical measurements and by antibody labeling. Because gap junctions can be regulated by second messenger-dependent mechanisms, and because the same intracellular messengers that are elevated during odor transduction can activate these mechanisms, coupling of olfactory neurons to other cells in the olfactory epithelium is expected to be modulated during odor transduction. Changes in coupling could affect odor sensitivity. Studies are proposed (1) to examine the extent of intercellular coupling in the epithelium using dye-transfer with Neurobiotin(TM) and Lucifer yellow, (2) to identify the subtypes of gap junction proteins in the olfactory epithelium and their distributions by cloning and the use of molecular techniques, and (3) to evaluate the pathways that regulate olfactory gap junctions using dye-tracer and electrophysiological methods. Gap junctions are now known to be common throughout the nervous system and to have important functional consequences, but our understanding of their physiological roles is poor. These studies should provide greater knowledge of both the olfactory system and of gap junctions, a basic interneuronal feature that occurs throughout the central nervous system.

本研究旨在识别和表征 嗅觉上皮间隙连接的生理作用。新的 数据表明，嗅觉受体神经元与一个 另一种可能是嗅上皮中的非神经元细胞 Necturus maculosus 的。通过染料转移揭示了偶联， 电测量和抗体标记。因为间隙连接 可以通过第二信使依赖机制进行调节，并且因为 在气味过程中升高的相同细胞内信使 转导可以激活这些机制，嗅觉神经元的耦合 嗅觉上皮中的其他细胞预计会受到调节 在气味传导过程中。耦合的变化可能会影响气味 敏感性。建议进行研究 (1) 来检验 使用染料转移在上皮中进行细胞间偶联 Neurobiotin(TM) 和 Lucifer Yellow，(2) 识别间隙亚型 嗅觉上皮中的连接蛋白及其分布 克隆和分子技术的使用，以及（3）评估 使用染料示踪剂调节嗅觉间隙连接的途径 电生理学方法。现在已知间隙连接很常见 整个神经系统并具有重要的功能 后果，但我们对其生理作用的了解还很有限。 这些研究应该提供对嗅觉和嗅觉的更多了解。 系统和间隙连接，这是发生的基本神经元特征 整个中枢神经系统。