REGULATION OF THE DEVRS OPERON IN MYXOCOCCUS XANTHUS

由于其在黄色粘球菌子实体中的关键作用 DevRS操纵子的形成及其作为遗传开关的潜在作用 将进一步分析，强调负自动调节。这个 自我调节蛋白将首先通过在 识别和确定哪一个基因影响积累 核糖核酸。然后，将确定监管是否处于 RNA的稳定性或转录水平的抑制。核糖核酸 稳定性将通过确定RNA在 野生型和突变株。如果这些菌株没有不同的话， 然后，我们将研究转录抑制。启动子区域 将首先进行测序，转录起点将是 利用S1作图和引物延伸进行鉴定。然后是一种突变 将进行启动子区域的分析以确定位置 是镇压所必需的。在此之后，自我调节蛋白将是 利用当前的融合蛋白技术进行纯化及其与 将对RNA或DNA进行分析。

Because of its critical role in Myxococcus xanthus fruiting body formation and its potential role as a genetic switch, the devRS operon will be further analyzed, emphasizing the negative autoregulation. The autoregulatory protein will first be identified by making deletions in the genes identified and determining which one affects the accumulation of RNA. Then, it will be determined if the regulation is at the level of RNA stability or at the level of repression of transcription. The RNA stability will be examined by determining the half-life of the RNA in the wild type and mutant strains. If it is not different in these strains, then repression of transcription will be examined. The promoter region will first be sequenced and the transcriptional start site will be determined using S1 mapping and primer extension. Then a mutational analysis of the promoter region will be performed to determine sites required for repression. After this, the autoregulatory protein will be purified using current fusion protein technology and interactions with the RNA or DNA will be analyzed.