Mechanisms Driving Emergent Behaviors during Myxococcus xanthus Development
黄粘球菌发育过程中驱动突发行为的机制
基本信息
- 批准号:1951025
- 负责人:
- 金额:$ 89.18万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-03-01 至 2024-08-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
How organisms build structures of controlled size and shape, and with cells that adopt alternative fates, are fundamental questions in biology. Myxococcus xanthus provides a unique opportunity to answer these questions: when starved, these rod-shaped bacteria move in streams and build mounds that produce structures, called fruiting bodies, while at the same time some cells differentiate into round spores. Other cells die or remain outside of the fruiting bodies as rods. Short-range signaling (called C-signaling) between cells coordinates streaming and mound formation with spore differentiation, but how this occurs is not fully known. To understand the process, the research will identify which genes are involved, how their expression changes during streaming and mound formation, what genes initiate change in cell shape, and how the cells communicate. Cell-cell interactions impact the life of all organisms. Further understanding of how cell-cell interactions drive behaviors will benefit society by, for example, providing information that will permit manipulation of microbial communities for pollution and climate control, and increase the potential for bioenergy and food production. The project will also benefit society by providing strong interdisciplinary training to students and by increasing public scientific literacy and engagement through interactive presentations and outreach events.Myxococcus xanthus is an attractive model system because multicellular development occurs rapidly and synchronously under laboratory conditions. The signaling and sensory capabilities of M. xanthus rival those of lower eukaryotes in complexity and include eukaryotic-like features, such as the use of short-range signaling to coordinate emergent behaviors. By focusing on how M. xanthus uses short-range signaling to coordinate streaming and mound formation with spore differentiation, the project will provide deep insight into emergent properties found commonly in development, such as assembly of multicellular structures and cell fate determination, using a highly genetically tractable system. The knowledge gained and methods developed will also catalyze research on microbial communities (microbiomes). The complexity of multispecies microbiomes and their environments makes it challenging to discover the underlying mechanisms and understand how they contribute to emergent properties. In particular, the role of short-range signaling is understudied. The research team's focus on how M. xanthus uses short-range signaling to drive successive emergent behaviors will advance knowledge that has potential to transform thinking about how the properties of microbiomes emerge. They will use a broad range of approaches including microscopy, genetics, biochemistry, and mathematical modeling. The team has extensive experience investigating signaling and gene regulation in bacteria, extracting data from microscopic images, and using data-driven computational simulations to interpret results and guide further experiments. Recently, they devised methods to visualize the shape and gene expression of individual cells during M. xanthus development. The new methodology paves the way for the work, which also relies on innovative strategies to identify genes driving emergent behaviors.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
生物体如何构建大小和形状可控的结构,以及采用另一种命运的细胞,是生物学中的基本问题。黄色粘球菌为回答这些问题提供了一个独特的机会:当饥饿时,这些杆状细菌在溪流中移动,建造土丘,产生被称为子实体的结构,同时一些细胞分化成圆形孢子。其他细胞死亡或保持在子实体外面的杆状。细胞间的短程信号(称为C-信号)与孢子分化协调流动和丘状形成,但这是如何发生的还不完全清楚。为了理解这一过程,这项研究将确定哪些基因参与其中,它们在分流和丘状形成过程中的表达如何变化,哪些基因启动了细胞形状的变化,以及细胞如何沟通。细胞间的相互作用影响着所有生物体的生命。进一步了解细胞-细胞相互作用如何驱动行为将使社会受益,例如,提供信息,允许操纵微生物群落来控制污染和气候,并增加生物能源和食品生产的潜力。该项目还将通过为学生提供强有力的跨学科培训,并通过互动演示和外联活动提高公众的科学素养和参与度,从而造福社会。黄色粘球菌是一个有吸引力的模型系统,因为多细胞发育在实验室条件下快速和同步发生。黄色微囊藻的信号和感觉能力在复杂性上与低等真核生物相媲美,并包括真核生物的类似特征,例如使用短程信号来协调紧急行为。通过重点研究黄曲霉如何利用短程信号来协调分流和土丘形成与孢子分化,该项目将利用一个高度遗传易懂的系统,深入了解发育中常见的新特性,如多细胞结构的组装和细胞命运的决定。所获得的知识和开发的方法也将促进对微生物群落(微生物群)的研究。多物种微生物群及其环境的复杂性使人们很难发现潜在的机制并了解它们如何对新出现的特性做出贡献。特别是,短程信号的作用还没有得到充分的研究。研究小组将重点放在黄色微藻如何使用短程信号来驱动连续的紧急行为,这将促进有可能改变对微生物群特性如何出现的思考的知识。他们将使用广泛的方法,包括显微镜、遗传学、生物化学和数学建模。该团队在研究细菌中的信号和基因调控、从微观图像中提取数据以及使用数据驱动的计算模拟来解释结果和指导进一步的实验方面拥有丰富的经验。最近,他们设计了一种方法来可视化黄花莲发育过程中单个细胞的形状和基因表达。新的方法为这项工作铺平了道路,这项工作还依赖于创新战略来识别驱动紧急行为的基因。这一奖项反映了NSF的法定使命,并通过使用基金会的智力优势和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Lee Kroos其他文献
Who's the Boss? One-Way Conversations between Bacteria
- DOI:
10.1016/j.devcel.2009.07.021 - 发表时间:
2009-08-18 - 期刊:
- 影响因子:
- 作者:
Lee Kroos - 通讯作者:
Lee Kroos
Short-range C-signaling restricts cheating behavior during emMyxococcus xanthus/em development
短程 C 信号传导限制了黄色粘球菌发育过程中的欺骗行为
- DOI:
10.1128/mbio.02440-24 - 发表时间:
2024-10-09 - 期刊:
- 影响因子:4.700
- 作者:
Y. Hoang;Joshua Franklin;Yann S. Dufour;Lee Kroos - 通讯作者:
Lee Kroos
Substrate engagement by the intramembrane metalloprotease SpoIVFB
膜内金属蛋白酶 SpoIVFB 对底物的结合
- DOI:
10.1038/s41467-024-52634-6 - 发表时间:
2024-10-17 - 期刊:
- 影响因子:15.700
- 作者:
Melanie A. Orlando;Hunter J. T. Pouillon;Saikat Mandal;Lee Kroos;Benjamin J. Orlando - 通讯作者:
Benjamin J. Orlando
Transcriptomic analysis of Myxococcus xanthus csgA, fruA, and mrpC mutants reveals extensive and diverse roles of key regulators in the multicellular developmental process
- DOI:
10.1186/s12864-025-11417-z - 发表时间:
2025-04-08 - 期刊:
- 影响因子:3.700
- 作者:
Mark A. Farrugia;Ramya Rajagopalan;Lee Kroos - 通讯作者:
Lee Kroos
Lee Kroos的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Lee Kroos', 18)}}的其他基金
Network Governing Sporulation during Myxococcus Development
粘球菌发育过程中的孢子形成网络
- 批准号:
1411272 - 财政年份:2014
- 资助金额:
$ 89.18万 - 项目类别:
Standard Grant
C-Signal-Dependent Gene Expression in Myxococcus Xanthus
Xanthus 粘球菌中 C 信号依赖性基因表达
- 批准号:
0744343 - 财政年份:2008
- 资助金额:
$ 89.18万 - 项目类别:
Standard Grant
C-Signal-Dependent Gene Expression in Myxococcus Xanthus
Xanthus 粘球菌中 C 信号依赖性基因表达
- 批准号:
0416456 - 财政年份:2004
- 资助金额:
$ 89.18万 - 项目类别:
Continuing Grant
C Signal-Dependent Gene Expression in Myxococcus xanthus
黄色粘球菌中 C 信号依赖性基因表达
- 批准号:
0090478 - 财政年份:2001
- 资助金额:
$ 89.18万 - 项目类别:
Continuing Grant
相似海外基金
DESIGN: Driving Culture Change in a Federation of Biological Societies via Cohort-Based Early-Career Leaders
设计:通过基于队列的早期职业领袖推动生物协会联盟的文化变革
- 批准号:
2334679 - 财政年份:2024
- 资助金额:
$ 89.18万 - 项目类别:
Standard Grant
CRII: RI: Deep neural network pruning for fast and reliable visual detection in self-driving vehicles
CRII:RI:深度神经网络修剪,用于自动驾驶车辆中快速可靠的视觉检测
- 批准号:
2412285 - 财政年份:2024
- 资助金额:
$ 89.18万 - 项目类别:
Standard Grant
Driving Behaviour in Multi-Winner Elections (BMW)
多位获胜者选举中的驾驶行为(宝马)
- 批准号:
EP/X038548/1 - 财政年份:2024
- 资助金额:
$ 89.18万 - 项目类别:
Research Grant
Driving Behaviour in Multi-Winner Elections (BMW)
多位获胜者选举中的驾驶行为(宝马)
- 批准号:
EP/X038351/1 - 财政年份:2024
- 资助金额:
$ 89.18万 - 项目类别:
Research Grant
The role of microbes in driving productivity and carbon fixation in seaweeds
微生物在提高海藻生产力和碳固定方面的作用
- 批准号:
2329475 - 财政年份:2024
- 资助金额:
$ 89.18万 - 项目类别:
Standard Grant
Near-infrared GaN quantum cascade laser for the next-generation self-driving car
用于下一代自动驾驶汽车的近红外GaN量子级联激光器
- 批准号:
23K20955 - 财政年份:2024
- 资助金额:
$ 89.18万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Collaborative Research: Understanding the impacts of an ongoing megadrought: Synthesizing the role of soil moisture in driving ecosystem fluxes from site to regional scales
合作研究:了解正在进行的特大干旱的影响:综合土壤湿度在驱动生态系统通量从场地到区域尺度方面的作用
- 批准号:
2331163 - 财政年份:2024
- 资助金额:
$ 89.18万 - 项目类别:
Standard Grant
I-Corps: Safe Driving Routing Tool
I-Corps:安全驾驶路线工具
- 批准号:
2349882 - 财政年份:2024
- 资助金额:
$ 89.18万 - 项目类别:
Standard Grant
Driving Innovation Responsibly: Commercialising CAV Services in the Scottish Highlands and Islands
负责任地推动创新:在苏格兰高地和岛屿实现 CAV 服务的商业化
- 批准号:
10085345 - 财政年份:2024
- 资助金额:
$ 89.18万 - 项目类别:
Legacy Department of Trade & Industry
SWEET SUCCESS: Driving net zero transition by accelerating bio-energy (sugarcane) production in South Africa
甜蜜的成功:通过加速南非生物能源(甘蔗)生产推动净零转型
- 批准号:
10104991 - 财政年份:2024
- 资助金额:
$ 89.18万 - 项目类别:
Demonstrator