MOLECULAR STRUCTURE OF ENDOSOMES IN DEVELOPING INTESTINE

发育中小肠内体的分子结构

• 批准号: 2395327
• 负责人: Jean M Wilson
• 金额: $ 18.09万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2395327
• 关键词: biomarker; endocytosis; gastrointestinal absorption /transport; gastrointestinal epithelium; glycoprotein structure; glycoproteins; growth /development; immunocytochemistry; immunoelectron microscopy; intracellular membranes; intracellular transport; laboratory rat; membrane structure; molecular cloning; newborn animals; nucleic acid probes; protein sequence; tissue /cell culture; transfection; vesicle /vacuole

DESCRIPTION: This is a renewal application to continue to study the development of absorptive function within the gastrointestinal tract in the neonatal rat. The enterocyte during early neonatal period has an elaborate endosomal system that has the capacity to endocytosis increase quantities of macromolecules. The intestinal tract also has a capacity for these engulfed vesicles to selectively transport physiologic molecules such as growth factors and hormones through the cell without disruption and either act directly on the cell or extended intact from the basolateral membrane. Alternatively, the enterocyte can function as a mucosal barrier to prevent the uptake of pathologic antigens and micro-organisms. The principal investigator has identified a molecule, endotubin, which is a specific protein present in the developing intestine within the apical endosome and can be used as a marker to define the function of apical endosomes in the developing intestine and to determine the process by which the endosome functions within the gastrointestinal tract. The goals of this current proposal, having cloned the endotubin DNA and identified its effect on non-polarized and polarized cell, is to: 1) identify the targeting signals involved in the biogenesis of specialized endosomes. 2) to define endocytosis and recycling through this compartment. 3) to isolate new endosomal proteins. 4) to map the chromosome localization of the human endotubin gene within the human genome. If these goals are accomplished, a better understanding of the importance of the epithelium as a barrier to the luminal environment will be forthcoming and ways to use the endosomal trafficking pattern appropriately in the delivery of either antibodies or physiologic molecules can be better understood.

描述：这是一个更新的应用程序，以继续研究 胃肠道内吸收功能的发展 新生大鼠 新生早期肠上皮细胞具有复杂的 具有内吞作用能力的内体系统增加了 大分子 肠道也有一个能力，这些吞噬 选择性运输生理分子的囊泡 因子和激素通过细胞而不受干扰， 直接位于细胞上或从基底外侧膜完整延伸。 或者，肠上皮细胞可以作为粘膜屏障，以防止 病理抗原和微生物的摄取。 校长 研究人员已经鉴定出一种分子，即内管蛋白， 存在于顶端内体内的发育肠中的蛋白质， 可以用作标记物来定义顶内体在细胞中的功能。 肠的发育，并确定内体 在胃肠道内发挥作用。 当前的目标 建议，克隆了内管蛋白DNA，并确定了其对 非极化和极化细胞，是：1）识别靶向信号 参与了专门的内体的生物发生。 2)以限定 内吞作用和通过该隔室的再循环。 3)分离新的 内体蛋白 4)绘制人类染色体定位图 内管蛋白基因。 如果实现这些目标， 更好地理解上皮作为屏障的重要性， 管腔环境将是即将到来的， 运输模式适当的抗体或 可以更好地理解生理分子。