PROTEINS INVOLVED IN REPLICATION OF HSV DNA

单纯疱疹病毒I型（HSV-I）是一种普遍存在的病原体， 急性和潜伏感染的大多数人口的 美国的 HSV的裂解周期是一个协调的结果， 病毒特异性蛋白质合成的时间级联 病毒DNA进入宿主细胞核 HSV主要DNA结合 蛋白质，通常称为ICP 8，是必需的或参与 病毒DNA的复制和病毒基因表达的调节。 在 在执行这些功能时，ICP 8与各种病毒编码的 和细胞大分子。 本提案的总体目标是确定和描述 ICP 8中的功能域。 实现这一目标的具体目标 目的如下： L. ICP 8锌结合结构域的定位和作用的确定 锌在ICP 8中的作用 2. ICP 8 DNA结合位点的定位及特异性DNA结合位点的鉴定 参与这种相互作用。 3.协作绑定所需域的标识和映射 ICP 8与核酸的结合和/或参与核酸内的构象变化 蛋白质。 4.识别和功能分析ICP 8区域， HSV DNA的来源依赖性合成。

Herpes Simplex Virus type l (HSV-l) is a ubiquitous pathogen which causes acute and latent infections in the majority of the population of the United States. The lytic cycle of HSV is the result of a coordinated temporal cascade of virus-specific protein synthesis following entry of the viral DNA into the host cell nucleus. The HSV major DNA-binding protein, commonly designated ICP8, is required for or involved in replication of the viral DNA and regulation of viral gene expression. in carrying out these functions ICP8 interacts with a variety of virus-coded and cellular macromolecules. The overall aim of this proposal is to identify and characterize functional domains within ICP8. The specific aims to accomplish this purpose are as follows: l. Mapping of the ICP8 zinc-binding domain and determination of the role of zinc in the functions of ICP8. 2. Mapping of the DNA binding-site of ICP8 and identification of specific residues involved in this interaction. 3. Identification and mapping of domains required for cooperative binding of ICP8 to nucleic acids and/or involved in conformational changes within the protein. 4. Identification and functional analysis of regions of ICP8 required for the origin-dependent synthesis of HSV DNA.