YEAST GENES IN RNA PROCESSING & NUCLEUS/CYTOSOL EXCHANGE
RNA 加工中的酵母基因
基本信息
- 批准号:2389488
- 负责人:
- 金额:$ 26.8万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1979
- 资助国家:美国
- 起止时间:1979-09-01 至 2001-06-30
- 项目状态:已结题
- 来源:
- 关键词:RNA biosynthesis RNA splicing Saccharomyces cerevisiae biological signal transduction cell nucleus cytoplasm enzyme activity fluorescence microscopy fungal genetics gene expression gene mutation guanosinetriphosphatase activating protein immunofluorescence technique in situ hybridization intracellular transport messenger RNA posttranscriptional RNA processing protein structure function ribosomal RNA stress proteins transfer RNA yeast two hybrid system
项目摘要
Eukaryotic cells are characterized by organelles, sites of
biochemical specialization. Nucleus/cytosol exchange is essential
for all eukaryotic organisms as RNAs are synthesized in the nucleus
and are transported to the cytosol where they function in protein
synthesis and many proteins synthesized in the cytosol function in
the nucleus in processes such as mitosis, DNA replication, and
RNA synthesis. The powerful yeast genetic system will be
employed to study the mechanism(s) controlling nucleus/cytosol
exchange and how exchange is coupled to RNA processing. Aim 1.
To test the hypothesis that Rna1p participates directly in mRNA
nuclear export. The Ran-GTPase cycle is required for nuclear
import and export and RNA processing, but the cellular distribution
of Rnalp, a regulatory protein of this cycle, raises questions as to
whether it has a direct role in export. Aim 2. To identify gene
products that function in the Ran-independent pathway for nuclear
export of mRNAs encoding stress proteins (Hsp). Previous studies
showed that Hsp mRNAs exit the nucleus via a Ran-independent
path. In situ hybridization will be used to identify mutants
defective in nuclear export of Hsp mRNAs. Aim 3. To test the
hypothesis that tRNA nuclear export is Ran-independent in yeast
and to identify the gene products that function in tRNA nuclear
export. Others have shown that in mammalian cells tRNAs exit the
nucleus via a Ran-independent pathway. In situ hybridization and
the 3-hybrid technique will be used to study the nucleus/cytosol
distribution of yeast precursor and mature tRNAs and to identify
mutants with altered distribution. Aim 4. To distinguish between
roles of Reg1p in regulating the Ran-GTPase cycle components or a
Ran-independent path of nucleus/cytosol exchange. Reg1 is a
suppressor of an RNA1 allele. To determine whether Reg1p
modulates the activities of the Ran-GTPase cycle components, their
subcellular locations or, instead, regulates Ran-independent
pathways, a combination of genetics, immunofluorescence, in situ
hybridization and biochemical assays will be employed. Aim5. To
test the hypothesis that the Ran-GTPase pathway functions in
processes other than nucleus/cytosol exchange. The Ran pathway
has been implicated in a variety of cellular processes in addition to
nucleus/cytosol exchange. The PI's lab generated an allele of the
gene encoding the Ran GAP that appears not to affect
nucleus/cytosol exchange, but rather affects cell cycle progression.
To test the model that this GAP participates in other cellular
processes, second-site and multicopy suppressors of this mutant
allele will be studied and the proteins that interact with Rna1p will
be identified by use of the 2-hybrid technique.
真核细胞的特点是细胞器,位点
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Anita K Hopper其他文献
Anita K Hopper的其他文献
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{{ truncateString('Anita K Hopper', 18)}}的其他基金
tRNA processing and nuclear-cytoplasmic dynamics
tRNA 加工和核质动力学
- 批准号:
9920190 - 财政年份:2017
- 资助金额:
$ 26.8万 - 项目类别:
tRNA processing and nuclear-cytoplasmic dynamics
tRNA 加工和核质动力学
- 批准号:
9284086 - 财政年份:2017
- 资助金额:
$ 26.8万 - 项目类别:
tRNA processing and nuclear-cytoplasmic dynamics
tRNA 加工和核质动力学
- 批准号:
10473791 - 财政年份:2017
- 资助金额:
$ 26.8万 - 项目类别:
tRNA processing and nuclear-cytoplasmic dynamics
tRNA 加工和核质动力学
- 批准号:
10296430 - 财政年份:2017
- 资助金额:
$ 26.8万 - 项目类别:
YEAST GENES IN RNA PROCESSING & NUCLEUS/CYTOSOL EXCHANGE
RNA 加工中的酵母基因
- 批准号:
7907380 - 财政年份:2009
- 资助金额:
$ 26.8万 - 项目类别:
MUTATIONS AFFECTING THE PRODUCTION OF MATURE RNAS
影响成熟 RNA 产生的突变
- 批准号:
3275156 - 财政年份:1979
- 资助金额:
$ 26.8万 - 项目类别:
MUTATIONS AFFECTING THE PRODUCTION OF MATURE RNAS
影响成熟 RNA 产生的突变
- 批准号:
3275164 - 财政年份:1979
- 资助金额:
$ 26.8万 - 项目类别:
MUTATIONS AFFECTING THE PRODUCTION OF MATURE RNAS
影响成熟 RNA 产生的突变
- 批准号:
3275161 - 财政年份:1979
- 资助金额:
$ 26.8万 - 项目类别:
YEAST GENES IN RNA PROCESSING & NUCLEUS/CYTOSOL EXCHANGE
RNA 加工中的酵母基因
- 批准号:
7148140 - 财政年份:1979
- 资助金额:
$ 26.8万 - 项目类别:
YEAST GENES IN RNA PROCESSING & NUCLEUS/CYTOSOL EXCHANGE
RNA 加工中的酵母基因
- 批准号:
7477593 - 财政年份:1979
- 资助金额:
$ 26.8万 - 项目类别:
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