FUNCTIONAL REGULATION OF DOPAMINE SIGNAL TRANSDUCTION

多巴胺信号转导的功能调节

• 批准号: 2460661
• 负责人: ASHIWEL S UNDIEH
• 金额: $ 13.56万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 2000-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2460661
• 关键词: G protein; adenylate cyclase; autoradiography; cyclic AMP; dopamine; dopamine agonists; dopamine antagonists; dopamine receptor; enzyme activity; gene deletion mutation; genetically modified animals; immunoprecipitation; laboratory mouse; laboratory rat; neural plasticity; neuropharmacology; phosphatidylinositols; phospholipase C; protein structure function; quinoline; receptor binding; receptor coupling; regulatory gene; reserpine

DESCRIPTION: (Applicant's abstract) This application is focussed on the signal transduction of dopamine. Recently, the applicant and several other investigators have demonstrated that some actions of dopamine in the brain may be mediated through the phosphoinositide signaling cascade rather than the classic adenylyl cyclase system. The goal of the proposed project will be to elucidate the mode and mechanisms of functional regulation in the phospholipase C coupled dopamine system. There are three primary questions to be addressed by the experiments: (1) Where in the brain does dopamine signal through the phospholipase C pathway?; (2) Which G-proteins mediate dopamine receptor coupling to phospholipase C?, and (3) How is the efficacy of dopamine receptor coupling to phospholipase C regulated?

描述：（申请人的摘要）本申请集中于 多巴胺信号转导 最近，申请人和其他几个 研究人员已经证明多巴胺在大脑中的某些作用 可能是通过磷酸肌醇信号级联介导的，而不是 经典的腺苷酸环化酶系统。 拟议项目的目标将 旨在阐明细胞功能调节的模式和机制， 磷脂酶C偶联多巴胺系统。 有三个主要问题 实验要解决的问题：（1）多巴胺在大脑中的什么位置 信号通过磷脂酶C途径？(2)哪些G蛋白介导 多巴胺受体与磷脂酶C？偶联，（3）疗效如何 多巴胺受体与磷脂酶C偶联的调节？