FUNCTIONAL REGULATION OF DOPAMINE SIGNAL TRANSDUCTION

描述：(申请者摘要)本申请的重点是 多巴胺的信号转导。最近，申请人和其他几个人 研究人员已经证明，大脑中的多巴胺的一些行为 可能是通过肌醇磷脂信号级联而不是 经典的腺酰环化酶系统。拟议项目的目标将是 目的是阐明脑缺血再灌注损伤的功能调节模式和机制。 磷脂酶C偶联的多巴胺系统。主要有三个问题 这些实验要解决的问题是：(1)多巴胺在大脑的什么地方 通过磷脂酶C途径传递信号？；(2)哪些G蛋白介导 多巴胺受体偶联到磷脂酶C？，以及(3)疗效如何 多巴胺受体与磷脂酶C偶联的调控？

DESCRIPTION: (Applicant's abstract) This application is focussed on the signal transduction of dopamine. Recently, the applicant and several other investigators have demonstrated that some actions of dopamine in the brain may be mediated through the phosphoinositide signaling cascade rather than the classic adenylyl cyclase system. The goal of the proposed project will be to elucidate the mode and mechanisms of functional regulation in the phospholipase C coupled dopamine system. There are three primary questions to be addressed by the experiments: (1) Where in the brain does dopamine signal through the phospholipase C pathway?; (2) Which G-proteins mediate dopamine receptor coupling to phospholipase C?, and (3) How is the efficacy of dopamine receptor coupling to phospholipase C regulated?