MECHANISM OF INITIATION BY RNA POLYMERASE II

RNA 聚合酶 II 的引发机制

• 批准号: 2415184
• 负责人: JAY D GRALLA
• 金额: $ 17.68万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2415184
• 关键词: DNA directed RNA polymerase; adenosine triphosphate; animal genetic material tag; enzyme activity; genetic promoter element; genetic transcription; helicase; messenger RNA; phosphorylation; transcription factor

The long-term goal of this work is to learn how mammalian cells produce levels of m-RNA appropriate to their specialized functions. This will be accomplished by investigating the roles of basal and activated transcription factors in setting m-RNA levels. The results will contribute to learning how mutant factors lead to inappropriate m-RNA production, a component of the imbalances that are associated with cancer. The specific aims of this project are: 1. To learn the relationships among the critical ATP-dependent steps occurring at polymerase II promoters. These include start site DNA opening, polymerase phosphorylation and transcription initiation. 2. To learn what mechanistic features limit the ability to transcribe a polymerase II promoter. This includes learning the roles of specific factors and elements in setting both the rate and efficiency of transcription initiation and re-initiation. 3. To learn to what extent the principles discovered in aims l and 2 might be generally applicable. This includes comparing selected promoters, with emphasis on TATA versus initiator and basal versus activated.

这项工作的长期目标是了解哺乳动物细胞如何产生 M-RNA的水平适合其专业功能。这会 可以通过研究基础和激活的作用来完成 设定M-RNA水平的转录因子。结果将 有助于学习突变因素如何导致不适当的M-RNA 生产，与之相关的失衡的组成部分 癌症。该项目的具体目的是： 1。了解关键ATP依赖性步骤之间的关系 发生在聚合酶II启动子上。这些包括开始站点DNA 开放，聚合酶磷酸化和转录起始。 2。了解哪些机械特征限制了转录的能力 聚合酶II启动子。这包括学习特定的角色 设定速率和效率的因素和要素 转录启动和重新调整。 3。要学习目标l和2中发现的原则在多大程度上 通常适用。这包括比较选定的 发起人，重点是塔塔与启动者，基础与基础与基础 活性。