课题基金 / 基金详情

DEAFNESS GENE

DEAFNESS GENE
耳聋基因
批准号：
2749257
负责人：
David C Kohrman
金额：
$10.64万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
1996
资助国家：
美国
项目状态：
已结题
起止时间：
1996-08-01 至 2001-07-31
来源：
https://reporter.nih.gov/project-details/2749257
关键词：
animal genetic material tag autosomal recessive trait deafness family genetics gene expression gene mutation gene rearrangement genetic disorder genetic library genetic mapping immunocytochemistry in situ hybridization laboratory mouse linkage mapping molecular cloning nucleic acid sequence polymerase chain reaction pulsed field gel electrophoresis sensorineural hearing loss single strand conformation polymorphism southern blotting

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英文摘要
DESCRIPTION (Adapted from the Investigator's Abstract): The objective of this proposal is to isolate and characterize the gene responsible for autosomal recessive deafness in the mouse pirouette mutant. Preliminary work has localized the gene to a 5 cM region of mouse chromosome 5 that is syntenic to 4p14-q12. Here Dr. Kohrman proposes to refine the map of the pirouette locus, evaluate candidate genes, construct a contig of the nonrecombinant region, and identify genes within the region by exon trapping and cDNA selection. Once a likely gene has been identified, it will be compared in structure and expression in control and mutant mice, and expression of the gene will be examined by ribonuclease protection, RT-PCR, and in situ hybridization. Finally, the human homolog will be cloned, sequenced, and chromosomally mapped in order to generate reagents for mutation detection in human patients.
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Protection from Noise Induced Hearing Loss - Genetic and Drug Induction of HSPs
Protection from Noise Induced Hearing Loss - Genetic and Drug Induction of HSPs
Mouse Deafness and Study of a Mouse Deafness Gene
Identification and Functional Analysis of the Mouse Deaf