MICROSURGICAL INVESTIGATION OF GASTRIC ACID SECRETION

胃酸分泌的显微外科研究

• 批准号: 2017043
• 负责人: John Peter GEIBEL
• 金额: $ 24.58万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 2000-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2017043
• 关键词: acidity /alkalinity; apical membrane; calcium channel blockers; calcium flux; calcium ion; enzyme activity; fluorescent dye /probe; gastric acid; histamine receptor; hormone regulation /control mechanism; hydrogen potassium exchanging ATPase; inositol phosphates; laboratory rabbit; membrane transport proteins; microsurgery; potassium channel; protein kinase A; protein kinase C; second messengers; secretion; sodium ion; sodium potassium exchanging ATPase; tissue /cell culture

DESCRIPTION: (Adapted from investigator's abstract) It is well known that the stomach plays a critical role in the digestion of foodstuffs taken in by the body by generating a solution that contains 0.16N hydrochloric acid. This role is essential for the normal survival of the organism. The conductive movement of H+ ions across the apical membrane plays a key role in acid secretion. The coupling of the net efflux of H+ ions with the recycling of K+ ions allows us to maintain the continued acid flux that occurs during stimulated acid secretion. In addition to this movement one expects to observe a parallel efflux of chloride to aid in the generation of acid from the gland lumen. The stimulated loss of acid occurs when the H-K-ATPase is actively inserted into the apical membrane causing a proton efflux from cell to lumen of the gland. What happens to the cells of the gland during the non-stimulated state is completely unknown due to the previous inaccessibility to the apical surface of the intact gland. The key focus of this grant will be to use the various techniques that have been developed in our laboratory to access the apical surface of the intact gland and to examine the biophysics and regulation of acid secretion transcellularly, focusing on the changes that develop from the non-stimulated gland to the stimulated gland. We will use our findings to explain what changes are developing in the stomach during both hyper- and hypoacidic states and to examine which of the major ions (Na+, Ca2+, K+, Cl-, HCO3) regulate, and/or stimulate the various phases of acid secretion. The results of this study will allow us to develop a new model of acid secretion including newly defined transport proteins that were inaccessible before the development of our various techniques. These data will help us to design strategies of control for both hyper- and hypoacidity.

描述：（改编自研究者的摘要）众所周知， 胃在消化食物中起着至关重要的作用 通过产生含有 0.16N 盐酸的溶液来进入体内。 这种作用对于生物体的正常生存至关重要。 这 H+离子穿过顶膜的传导运动起着关键作用 在酸分泌中。 H+ 离子的净流出与 K+ 离子的回收使我们能够保持持续的酸通量， 发生在刺激酸分泌期间。 除了这个动作之外还有一个 期望观察到氯化物的平行流出，以帮助产生 来自腺腔的酸。 当以下情况发生时，会发生刺激性的酸损失： H-K-ATP酶主动插入顶膜，产生质子 从细胞流出到腺体腔。 的细胞会发生什么 腺体在非刺激状态下是完全未知的，因为 以前无法到达完整腺体的顶端表面。 关键 这笔赠款的重点将是使用已被采用的各种技术 我们实验室开发的用于进入完整腺体的顶端表面 并检查酸分泌的生物物理学和调节 跨细胞，重点关注从 未受刺激的腺体到受刺激的腺体。 我们将利用我们的发现 解释在过度和过度状态下胃部发生的变化 低酸状态并检查主要离子（Na+、Ca2+、K+、 Cl-、HCO3)调节和/或刺激酸分泌的各个阶段。 这项研究的结果将使我们能够开发一种新的酸模型 分泌包括新定义的无法接近的转运蛋白 在我们开发各种技术之前。 这些数据将帮助我们 设计控制胃酸过多和胃酸过少的策略。