ROLE OF DIFFERENTIATION FACTORS IN CARTILAGE AND BONE FORMATION AND REGENERATION

这个项目的目标是研究软骨和骨骼。 并确定它们在胚胎发生和发育中的作用。 出生后的生命，包括组织形成和疾病。作为组织 再生概括了胚胎的发育顺序 可以想象，了解组织形成的机制 可溶性分化因子的作用是关键的一步 以生物控制的方式再生骨骼组织。 这将对先天性心脏病的治疗产生重大影响。 和/或获得性骨骼疾病，如大骨缺陷症， 骨折愈合障碍、骨关节炎、骨质疏松症和 牙周炎。这个项目的重点是进一步的表征 软骨和骨诱导分子，转化生长因子-β的成员 超家族以及它们的生物学活动。使用分子 探针，我们正在研究它们对软骨的各自贡献 软骨内和膜性骨形成。免疫组织化学 软骨和骨诱导的定位和原位杂交 蛋白质，以及体外和体内的研究表明， 这些分子对启动、增强、 软骨细胞和成骨细胞的维持和成熟 表型。分子遗传学方法揭示了生理上的 这些形态因子在骨骼发育中的作用。

The objectives of this project are to study the cartilage and bone inducing factors and to define their role in embryogenesis and in postnatal life, both in tissue formation and in disease. As tissue regeneration recapitulates the developmental sequence of embryonic tissue formation, it is conceivable that understanding the mechanisms of action of the soluble differentiation factors is a key step towards biologically controlled regeneration of skeletal tissues. This will have a significant impact on the treatment of congenital and/or acquired skeletal diseases such as large bone defects, impaired fracture healing, osteoarthritis, osteoporosis and periodontitis. This project focuses on the further characterization of cartilage and bone inducing molecules, members of the TGF-B superfamily, and their biological activities. Using molecular probes, we are studying their respective contributions to cartilage and endochondral and membranous bone formation. Immunohistochemical localization and in situ hybridization of cartilage and bone inducing proteins, as well as studies in vitro and in vivo, indicate the selective contribution of these molecules to initiation, enhancement, maintenance and maturation of the chondrocytic and osteoblastic phenotypes. Molecular genetic approaches reveal the physiological roles of these morphogens in skeletal development.