ROLE OF DIFFERENTIATION FACTORS IN CARTILAGE AND BONE FORMATION AND REGENERATION

本项目的目标是研究软骨和骨 诱导因子，并确定它们在胚胎发生和 在组织形成和疾病中的作用。 诸如组织 再生概括了胚胎组织的发育顺序 形成，可以想象，了解作用机制 可溶性分化因子的合成是生物学上 骨骼组织的受控再生。 这将是一个重大的 对先天性和/或后天性骨骼疾病治疗的影响 例如大的骨缺损、受损的骨折愈合、骨关节炎 骨质疏松症和牙周炎。 该项目的重点是进一步 软骨和骨诱导分子的表征， TGF-β超家族及其生物学活性。 使用分子 探针，我们正在研究它们各自对软骨的贡献， 软骨内骨和膜骨形成。 免疫组 软骨和骨诱导的定位和原位杂交 蛋白质，以及在体外的研究，表明选择性的贡献， 启动、增强、维持和成熟 软骨细胞和成骨细胞表型的区别

The objectives of this project are to study the cartilage and bone inducing factors and to define their role in embryogenesis and in postnatal life, both in tissue formation and in disease. As tissue regeneration recapitulates the developmental sequence of embryonic tissue formation, it is conceivable that understanding the mechanisms of action of the soluble differentiation factors is a key step towards biologically controlled regeneration of skeletal tissues. This will have a significant impact on the treatment of congenital and/or acquired skeletal diseases such as large bone defects, impaired fracture healing, osteoarthritis, osteoporosis and periodontitis. This project focuses on the further characterization of cartilage and bone inducing molecules, members of the TGF-B superfamily, and their biological activities. Using molecular probes, we are studying their respective contributions to cartilage and endochondral and membranous bone formation. Immunohistochemical localization and in situ hybridization of cartilage and bone inducing proteins, as well as studies in vitro, indicate the selective contribution of these molecules to initiation, enhancement, maintenance and maturation of the chondrocytic and osteoblastic phenotypes.