DETERMINATION OF 3 DIMENSIONAL STRUCTURES OF MACROMOLECULES IN SOLUTION BY NMR
核磁共振法测定溶液中大分子的三维结构
基本信息
- 批准号:2572979
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:
- 资助国家:美国
- 起止时间:至
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Work in this laboratory has been focussed on the determination of
three-dimensional structures of larger proteins in solution by NMR,
with a particular emphasis on protein-protein, protein-ligand and
protein-DNA complexes. A considerable effort has been placed on the
developments of three- and four-dimensional heteronuclear NMR to
extend the application of NMR as a method for determining three-
dimensional structures of proteins in solution beyond the limits of
conventional two-dimensional NMR (about 100 residues) to molecules in
the 150- to 400- residue range.
Solution structures of a number of proteins have been determined.
These include the complexes of the transcription factors GAGA, Are A
and HMG-I/Y with DNA, complexes of human thioredoxin with its target
site from Ref and NfkB, and the DNA binding domains of the transposase
and HIV-integrase-Streptococcal protein G have been investigated, and
the stability and structural integrity of a number of multiple core
mutants of Streptococcal protein G have been examined.
本实验室的工作重点是测定
通过核磁共振研究溶液中较大蛋白质的三维结构,
特别强调蛋白质-蛋白质、蛋白质-配体和
蛋白质-DNA复合物。 在这方面已经作出了相当大的努力,
发展三维和四维杂波核磁共振,
扩展了NMR作为测定三个-
蛋白质在溶液中的空间结构超出了
常规的二维NMR(约100个残基)对分子进行分析。
150- 400个残基范围。
一些蛋白质的溶液结构已被确定。
这些包括转录因子GAGA、Are A
和HMG-I/Y与DNA,人硫氧还蛋白与其靶标的复合物
来自Ref和NfkB的位点,以及转座酶的DNA结合结构域
和HIV-整合酶-链球菌蛋白G进行了研究,
多个多芯稳定性和结构完整性
已经检测了链球菌蛋白G的突变体。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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G. MARIUS CLORE其他文献
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{{ truncateString('G. MARIUS CLORE', 18)}}的其他基金
DETERMINATION OF 3 DIMENSIONAL STRUCTURES OF MACROMOLECULES IN SOLUTION BY NMR
核磁共振法测定溶液中大分子的三维结构
- 批准号:
6161936 - 财政年份:
- 资助金额:
-- - 项目类别:
DETERMINATION OF 3 DIMENSIONAL STRUCTURES OF MACROMOLECULES IN SOLUTION BY NMR
核磁共振法测定溶液中大分子的三维结构
- 批准号:
6432092 - 财政年份:
- 资助金额:
-- - 项目类别:
DETERMINATION OF 3 DIMENSIONAL STRUCTURES OF MACROMOLECULES IN SOLUTION BY NMR
核磁共振法测定溶液中大分子的三维结构
- 批准号:
6105207 - 财政年份:
- 资助金额:
-- - 项目类别:
DETERMINATION OF 3 DIMENSIONAL STRUCTURES OF MACROMOLECULES IN SOLUTION BY NMR
核磁共振法测定溶液中大分子的三维结构
- 批准号:
6289751 - 财政年份:
- 资助金额:
-- - 项目类别:
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