MOLECULAR REGULATION OF MAMMALIAN PREGNANCY DURATION

哺乳动物怀孕持续时间的分子调控

• 批准号: 2673933
• 负责人: CHARLES F SIMMONS
• 金额: $ 10.3万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-16 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2673933
• 关键词: RNase protection assay; biological signal transduction; estrogens; gel electrophoresis; gene expression; genetic regulatory element; genetically modified animals; hormone receptor; hormone regulation /control mechanism; immunocytochemistry; laboratory mouse; laboratory rabbit; molecular cloning; myometrium; nucleic acid sequence; oxytocin; polymerase chain reaction; pregnancy; receptor expression; regulatory gene; reporter genes; tissue /cell culture; transcription factor

DESCRIPTION (Adapted from the Investigator's Abstract): This application seeks to examine regulation of myometrial OTR expression in a murine model. Specifically, the investigator will 1) define the molecular organization and regulatory regions of the mouse OTR gene by genomic cloning, DNA sequence analysis, and identification of putative transcription factor consensus sequences; 2) test putative cis-acting OTR gene regulatory elements in vitro via quantitation of OTR-luciferase reporter gene expression in transfected primary myometrial myocytes and cell lines, and modulation by estrogenic steroids; and 3) define the genetic information required for spatial, temporal, and estrogen (E) regulation of the native OTR gene in transgenic mice that express an OTR-beta-galactosidase reporter gene.

描述（改编自研究者摘要）：本申请 试图在鼠模型中检查子宫肌层OTR表达的调节。 具体而言，研究者将1）定义分子组织， 通过基因组克隆、DNA序列测定， 分析和鉴定推定的转录因子共有序列 序列; 2）在体外测试推定的顺式作用OTR基因调控元件 通过定量转染的细胞中的OTR-荧光素酶报告基因表达， 原代子宫肌细胞和细胞系，以及雌激素调节 类固醇;和3）定义空间， 转基因小鼠中天然OTR基因的时间和雌激素（E）调节 表达OTR-β-半乳糖苷酶报告基因的小鼠。