ROLE OF CELL ADHESION IN BIOLOGICAL SIGNAL TRANSDUCTION
细胞粘附在生物信号转导中的作用
基本信息
- 批准号:6238317
- 负责人:
- 金额:$ 2.26万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1997
- 资助国家:美国
- 起止时间:1997-07-01 至 1998-06-30
- 项目状态:已结题
- 来源:
- 关键词:affinity chromatography biological signal transduction cell adhesion cell differentiation dental implants extracellular matrix fibroblasts immunofluorescence technique immunologic assay /test immunoprecipitation osteoblasts protein purification protein tyrosine phosphatase tissue /cell culture western blottings wound healing
项目摘要
Focal adhesions (FA) are prominent transmembrane
structures of many cells in culture. Individual FA's serve to link the
extracellular matrix (ECM) to the actin cytoskeleton, and to initiate a
cascade of signalling pathways through integrin receptors. The regulation
of these cellular signalling pathways is important with respect to many
cellular functions including growth and differentiation, tumorigenesis,
wound healing, osseointegration, and the formation of a mineralized
matrix. The objective of this study was to evaluate protein tyrosine
phosphatase (PTP) activity that may regulate tyrosine phosphorylation in
FA's. Using a PTP assay, we have confirmed the observations of Maher
(PNAS 90:11177, 1993) that lysates of fibroblasts in suspension have
elevated PTP activity when compared with lysates of adherent fibroblasts.
Looking for PTP's that may be adhesion regulated, we have identified the
cytoplamic PTP, PTPDl, in immunoprecipitates of the FA protein paxillin.
We are currently exploring this interaction and the relationship of this
PTP to FA's. Microinjection of the cytoplasmic domain of a receptor-type
PTP, PTP , (GST fusion protein subclone generously provided by Mathew
Thomas, Wash. Univ., St. Louis) into adherent fibroblasts depleted the
tyrosine phosphorylation in FA's. However, the FA structure appeared to
be stable as judged by staining with anti-vinculin. Our results suggest
that the tyrosine phoshorylation in FAs is regulated not only by kinases
but also by PTP's.
局灶性粘连(FA)是突出的跨膜
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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GALEN B SCHNEIDER其他文献
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{{ truncateString('GALEN B SCHNEIDER', 18)}}的其他基金
Mineralization: Integrins and Focal Adhesion Kinase
矿化:整合素和粘着斑激酶
- 批准号:
6625686 - 财政年份:2002
- 资助金额:
$ 2.26万 - 项目类别:
Mineralization: Integrins and Focal Adhesion Kinase
矿化:整合素和粘着斑激酶
- 批准号:
6478029 - 财政年份:2002
- 资助金额:
$ 2.26万 - 项目类别:
ROLE OF CELL ADHESION IN BIOLOGICAL SIGNAL TRANSDUCTION
细胞粘附在生物信号转导中的作用
- 批准号:
5210031 - 财政年份:
- 资助金额:
$ 2.26万 - 项目类别:
相似海外基金
ROLE OF CELL ADHESION IN BIOLOGICAL SIGNAL TRANSDUCTION
细胞粘附在生物信号转导中的作用
- 批准号:
5210031 - 财政年份:
- 资助金额:
$ 2.26万 - 项目类别: