MOLECULAR TYPING OF RECURRENT CHLAMYDIAL STDS

复发性衣原体标准的分子分型

• 批准号: 2004604
• 负责人: DEBORAH Anne DEAN
• 金额: $ 15.63万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2004604
• 关键词: Chlamydia trachomatis; adolescence (12-20); bacterial genetics; bacterial proteins; chlamydial disease; clinical research; epidemiology; genetic strain; genotype; human subject; membrane proteins; polymerase chain reaction; relapse /recurrence; serotyping; sexually transmitted diseases; sign /symptom

DESCRIPTION (Adapted from the applicant's abstract): Chlamydia trachomatis is the leading cause of sexually transmitted diseases (STD) in the developed world. The majority of these infections are subclinical and non-acute in presentation which accounts for the large number of women with tubal infertility and ectopic pregnancies that are so prevalent today. Further, up to 50% of adolescent and young adult women become reinfected with Chlamydia which contributes to tubal scarring. Thus, adolescents are an important target group in which to analyze transmission patterns and types of recurrence in order to design appropriate interventional strategies. Strain typing is essential for defining the molecular epidemiology of chlamydial infections within the context of different chlamydial asymptomatic and symptomatic disease states that include urethritis, cervicitis, pelvic inflammatory disease, and recurrent infection. Monoclonal antibodies to the major outer membrane protein (MOMP) of C. trachomatis have historically been used for typing. Yet, serotypes do not reflect the same degree of variation found at the gene and protein level of this organism. ompl genotyping has been developed which is based on the MOMP gene (ompl ). It is capable of detecting new subtypes of currently known serotypes and provides a powerful alternative to immunotyping. Genotyping is an appropriate tool for evaluating transmission patterns and recurrent infections, and for use as a biologic measure of behavior in behavioral related studies and for public health surveillance. Specific Aims: 1) define the ompl genotypes and variants among adolescent males and females who differ by force-of-infection and behavioral variables; 2) analyze transmission patterns of ompl genotypes between partners and within teen groups; 3) evaluate types of recurrent infections that occur in adolescents in comparison with first (naive) or previous infections, define types of mutations that occur in ompl over these time points, and evaluate the role of genotype polymorphism, co-pathogens, and immune response in reinfection at cervical and urethral sites; and 4) define molecular characteristics of genotypes that may be associated with clinical and microbiologic data; analyze the sequence-structure/function relationships of MOMP from prototype and variant strains.

性状（改编自申请人摘要）：沙眼衣原体 性传播疾病（STD）的主要原因是什么？ 世界 这些感染大多数是亚临床和非急性的， 介绍这占了大量的妇女与输卵管 不孕不育和宫外孕的问题。 此外，本发明还 高达50%的青少年和年轻成年妇女再次感染 衣原体，这有助于输卵管疤痕。 因此，青少年是一个 分析传播模式和类型重要目标群体 以设计适当的干预策略。 菌株分型对于确定大肠杆菌的分子流行病学至关重要。 不同衣原体感染背景下的衣原体感染 无症状和有症状的疾病状态包括尿道炎， 宫颈炎、盆腔炎、反复感染。 针对C.主要外膜蛋白（MOMP）的单克隆抗体。 沙眼在历史上被用于分型。 然而，血清型不 反映了在基因和蛋白质水平上发现的相同程度的变异， 这个有机体。 ompl基因分型已经开发出来，它是基于 MOMP基因（ompl）。它能够检测目前的新亚型， 已知的血清型，并提供了一个强大的替代免疫分型。 基因分型是评估传播模式的适当工具， 复发性感染，并用作行为的生物学测量， 行为相关研究和公共卫生监测。 具体目的：1）明确青少年ompl基因型及其变异 感染力和行为变量不同的男女; 2)分析伴侣之间ompl基因型的传播模式， 在青少年群体中; 3）评估发生在青少年群体中的复发性感染的类型 青少年与首次（首次）或既往感染相比，定义 在这些时间点发生在ompl中的突变类型，并评估 基因型多态性、共病原体和免疫应答在 宫颈和尿道部位的再感染;以及4）确定分子 基因型的特征可能与临床和 微生物数据;分析序列结构/功能关系 来自原型和变体菌株的MOMP。