TISSUE SPECIFIC TUMOR INDUCTION BY POLYOMAVIRUS
TISSUE SPECIFIC TUMOR INDUCTION BY POLYOMAVIRUS
批准号:
2330883
负责人:
Robert Freund
金额:
$9.91万
依托单位国家:
美国
项目类别:
财政年份:
1995
资助国家:
美国
项目状态:
已结题
起止时间:
1995-02-01 至 2000-01-31
关键词:
DNA binding protein DNA footprinting Polyomavirus affinity chromatography gel mobility shift assay gene mutation genetic enhancer element genetic regulatory element laboratory mouse nucleic acid sequence oligonucleotides oncogenic virus thymus neoplasms viral carcinogenesis virus genetics virus infection mechanism virus related neoplasm /cancer
中文摘要
多瘤病毒通常在脑脊液中形成沉默的、持续的感染
英文摘要
Polyomavirus normally establishes a silent, persistent infection in its
natural host, the mouse, but under experimental conditions can cause
widespread lytic infection and induce tumors in up to a dozen different
tissues, including a high frequency of tumors in thymic tissue, salivary
glands and mammary glands. Although the virus has been studied extensively
in cell culture, little is known about its interaction with host cells in
the animal. The experiments in this proposal are designed to explore the
virus-cell interactions that result in tissue specificity of tumor
formation by polyomavirus.
The two specific aims in this proposal are based on the hypothesis that
viral regulatory sequences interacting with cellular factors are
responsible for tissue specific tumor induction. The first goal is to
define the viral genetic determinants responsible for tissue specific
tumorigenesis by evaluating the tumor profile of viruses containing
mutations in potential regulatory sequences. These regulatory regions
include a 4Obp duplication of sequences on the early side of the origin of
replication that we have previously shown to be necessary for the
induction of thymic epitheliomas, and the polyoma enhancer sequences that
are important for virus regulation in cell culture systems. Our second
goal is to use these regulatory sequences to identify and characterize the
cellular factors that bind to them. The DNA binding proteins will be
identified by gel shift and footprinting experiments and further
characterized by UV crosslinking experiments and affinity chromatography.
Polyomavirus gene expression and replication, as well as the oncogenic
pathways triggered by the virus, utilize cellular factors that are
normally involved in signal transduction and cell growth control, and that
are altered in many cancers of non-viral etiology. Thus, understanding
tumor induction by polyomavirus at the molecular level and identifying
cis-acting sequences and cellular factors involved in the tissue
specificity of tumor formation will provide new information relevant to
the fields of cell growth control and cancer.
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TISSUE SPECIFIC TUMOR INDUCTION BY POLYOMAVIRUS
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批准号:2104750
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项目类别:
-
资助金额:$9.72万
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财政年份:1995
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负责人:Robert Freund
-
依托单位:
TISSUE SPECIFIC TUMOR INDUCTION BY POLYOMAVIRUS
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批准号:2654137
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项目类别:
-
资助金额:$10.31万
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财政年份:1995
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负责人:Robert Freund
-
依托单位:
TISSUE SPECIFIC TUMOR INDUCTION BY POLYOMAVIRUS
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批准号:2104751
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项目类别:
-
资助金额:$9.52万
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财政年份:1995
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负责人:Robert Freund
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依托单位:
THE ROLE OF THE HRT GENE OF POLYOMA IN LYTIC INFECTIONS
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批准号:3032294
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项目类别:
-
资助金额:$2.6万
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财政年份:1986
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负责人:Robert Freund
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依托单位:
THE ROLE OF THE HRT GENE OF POLYOMA IN LYTIC INFECTIONS
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批准号:3032293
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项目类别:
-
资助金额:$2.5万
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财政年份:1985
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负责人:Robert Freund
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依托单位:
海外基金