REGULATION OF TESTICULAR INHIBIN/ACTIVIN GENE EXPRESSION

睾丸抑制素/激活素基因表达的调节

• 批准号: 2016139
• 负责人: CHING-LING C CHEN
• 金额: $ 14.66万
• 依托单位: POPULATION COUNCIL
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2016139
• 关键词: Leydig cells; Sertoli cells; binding proteins; cell line; developmental genetics; gene expression; histogenesis; inhibin; laboratory rat; reproductive development; testis; transcription factor

DESCRIPTION (Adapted from the applicant's abstract): The goal of this research is to study the molecular mechanisms involved in controlling testicular physiology and male reproduction. Aim 1 will investigate the control of transcription of the rat inhibin a-subunit gene by GATA-binding proteins in MA-10 cells and rat Sertoli and Leydig cells, and will identify cofactors and cell-specific proteins that interact with GATA-1 to regulate the a-subunit gene. Aim 2 is to investigate the regulation of two promoters within the rat bB-subunit gene that give rise to two species (4.8- and 3.7-kb) of the bB-subunit mRNA from alternate initiation sites. Aim 3 will investigate the developmental expression and hormonal regulation of GATA-binding proteins in the rat testis to determine whether any observed changes correlate to the known regulation of the a- and bB-subunit genes.

描述（改编自申请人的摘要）：本研究的目标 研究的目的是研究参与控制的分子机制 睾丸生理学和男性生殖。目标1将调查 通过GATA结合调控大鼠胰高血糖素α-亚基基因的转录 蛋白质在MA-10细胞和大鼠睾丸支持细胞和Leydig细胞，并将确定 与加塔-1相互作用以调节 a亚基基因。目的二是研究两个启动子的调控 在大鼠bB亚基基因内，产生两种物种（4.8-和 3.7-kb）的bB-亚基mRNA的交替起始位点。目标3将 研究发育表达和激素调节 GATA结合蛋白，以确定是否有任何观察到的 这些变化与已知的α-和bB-亚基基因的调节相关。