DETECTION OF CAMPYLOBACTER BY PCR/IMMUNOASSAY SYSTEM

由弯曲杆菌引起的人类腹泻疾病是主要的 对公众健康的威胁，对第三世界的威胁 国家和较低的社会经济阶层，以及美国军方 部署在国外的人员。可用的详细的实验室诊断 目前还不太适合常规检测。最近的几个 研究已经开发出使用鞭毛蛋白基因的诊断工具 用聚合酶链式反应从粪便和水样中特异性检测细菌。这个 目前的方案使用抗鞭毛抗体涂层磁珠来 从粪便样本中捕获细菌。从这些捕获到的DNA中 细菌将被用作对鞭毛进行PCR扩增的模板 吉恩。使用简单的ELISA法的检测系统将使高- 吞吐量筛选。在标准化之后，整个程序应该 在不到10小时内完成。这种技术的巨大潜力 试剂盒在弯曲杆菌诊断中的应用是显而易见的，并可以 更换当前使用的诊断工具。 建议的商业应用：该项目有助于轻松、快速和 患者粪便标本中弯曲杆菌的特异性诊断 患有肠炎，具有特殊的应用价值。它提供了 作为弯曲杆菌诊断试剂盒的巨大商业应用 肠炎。

Diarrheal diseases caused by camplobacter species in humans pose a major threat to the health of the general public, to those in third world countries and of lower socioeconomic classes, and to U.S. military personnel deployed abroad. The elaborate laboratory diagnoses available at present are not well-suited for routine detection. A few recent studies have developed diagnostic tools using the flagellin gene for specific detection of bacteria from stool and water samples by PCR. The current proposal uses antiflagellar antibody coated magnetic beads to capture the bacteria from stool samples. The DNA from these captured bacteria will be used as a template for PCR amplification of the flagellar gene. A detection system using a simple ELISA assay will enable a high- throughput screening. After standardization, the entire procedure should be completed in less than 10 hours. The tremendous potential for such a kit is evident in its use for the diagnosis of campylobacter and could replace the currently used diagnostic tools. PROPOSED COMMERCIAL APPLICATION: This project aids in the easy, rapid and specific diagnosis of campylobacter in stool samples from patients suffering from enteritis and thus has exceptional application. It offers a tremendous commercial application as a diagnostic kit for campylobacter enteritis.