DETECTION OF CAMPYLOBACTER BY PCR/IMMUNOASSAY SYSTEM

通过 PCR/免疫分析系统检测弯曲杆菌

• 批准号: 2005313
• 负责人: TERESA A LEHMAN
• 金额: $ 9.45万
• 依托单位: BIOSERVE BIOTECHNOLOGIES, LTD
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2005313
• 关键词: Helicobacter; clinical research; communicable disease diagnosis; diagnosis design /evaluation; enteritis; enzyme linked immunosorbent assay; feces analysis; human subject; method development; polymerase chain reaction

Diarrheal diseases caused by camplobacter species in humans pose a major threat to the health of the general public, to those in third world countries and of lower socioeconomic classes, and to U.S. military personnel deployed abroad. The elaborate laboratory diagnoses available at present are not well-suited for routine detection. A few recent studies have developed diagnostic tools using the flagellin gene for specific detection of bacteria from stool and water samples by PCR. The current proposal uses antiflagellar antibody coated magnetic beads to capture the bacteria from stool samples. The DNA from these captured bacteria will be used as a template for PCR amplification of the flagellar gene. A detection system using a simple ELISA assay will enable a high- throughput screening. After standardization, the entire procedure should be completed in less than 10 hours. The tremendous potential for such a kit is evident in its use for the diagnosis of campylobacter and could replace the currently used diagnostic tools. PROPOSED COMMERCIAL APPLICATION: This project aids in the easy, rapid and specific diagnosis of campylobacter in stool samples from patients suffering from enteritis and thus has exceptional application. It offers a tremendous commercial application as a diagnostic kit for campylobacter enteritis.

由沙门氏菌引起的人类腹泻病是 对公众健康的威胁，对那些在第三世界 国家和较低的社会经济阶层，以及美国军方 部署在国外的人员。 现有的精密实验室诊断 目前还不太适合常规检测。 A few recent 研究已经开发出利用鞭毛蛋白基因诊断 通过PCR特异性检测粪便和水样中的细菌。 的 目前的建议使用抗鞭毛抗体包被的磁珠， 从粪便样本中捕获细菌。 从这些捕获的DNA中 将细菌用作鞭毛的PCR扩增的模板 基因 使用简单的ELISA测定的检测系统将能够实现高- 通量筛选 标准化后，整个程序应该 在不到10小时内完成。 这样一个巨大的潜力， 该试剂盒在其用于诊断弯曲杆菌方面是明显的， 更换目前使用的诊断工具。 建议的商业应用：该项目有助于简单，快速， 病人粪便标本中弯曲杆菌特异性诊断 患有肠炎，因此具有特殊的应用。 它提供 作为弯曲杆菌的诊断试剂盒， 肠炎。