SCREENING OF THERMOPHILES FOR ANTIFUNGAL ACTIVITY

描述：新型生物活性化合物的鉴定正在成为 越来越困难。已经筛选了许多潜在的来源 无数次，减少了找到额外的，有用的 材料.避免这个绊脚石的一种方法是识别小说 微生物的一个更富有成效的地方找到未经考验的 生物是寻找栖息地，大部分是未开发的，但已知的 包括活跃的微生物群落。本申请建议 筛选在热沃茨中微生物席中发现的有机体的提取物 以及来自于抗真菌活性的乙酰丙酮。 第一阶段申请的两个目标是：1。纳入 一组屏幕来测试样品的抗真菌活性。这些将 包括全细胞测定、体外酶筛选和OS-1细胞壁 屏幕; 2。测试嗜热微生物的抗真菌活性。 这项工作将确定产生抗真菌抗生素的生物体 从以前未屏蔽的环境中。很可能这些 化合物将是新颖的，并导致新的抗真菌治疗剂。

DESCRIPTION: The identification of novel bioactive compounds is becoming increasingly more difficult. Many potential sources have been screened numerous times, decreasing the possibility of finding additional, useful materials. One way to avoid this stumbling block is to identify novel microorganisms. One of the more fruitful places to find untested organisms is to search habitats that are largely unexplored but are known to include active microbial communities. This application proposes to screen extracts from organisms found in microbial mats in thermal waters and from fumaroles for antifungal activity. The two objectives in this Phase I application are: 1. To integrate a battery of screens to test samples for antifungal activity. These will include whole cell assays, in vitro enzyme screens and os-1 cell-wall screen; and 2. To test thermophilic organisms for antifungal activity. This work will identify organisms that produce antifungal antibiotics from previously unscreened environments. It is likely that these compounds will be novel and lead to new antifungal therapeutics.