MOLECULAR EPIDEMIOLOGY OF CYCLOSPORA

环孢子虫的分子流行病学

• 批准号: 2440143
• 负责人: RODNEY D ADAM
• 金额: $ 7.58万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-15 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2440143
• 关键词: diarrhea; egg /ovum; epidemiology; genetic library; genetic markers; microorganism genetics; molecular cloning; nucleic acid sequence; parasitic disease diagnosis; polymerase chain reaction; protozoa; protozoal infection; ribosomal DNA

DESCRIPTION (adapted from application abstract): Cyclospora cayetanensis is a newly recognized cause of endemic and epidemic diarrhea throughout the world. When the organism was first recognized in 1985, it was thought to be a protozoan (called crypto grande) or a cyanobacterium-like body (CLB) or a blue green alga, because of its autofluorescence. The organism was confirmed to be a coccidian at the University of Arizona in 1993 when it was induced to sporulate. Since then, Cyclospora continues to be recognized as an important cause of diarrhea and malabsorption in a number of developing countries. It also has been recognized as a cause of epidemic disease in the U.S. For example there was a well-publicized outbreak in 1996 involving Florida and 13 other states, possibly associated with imported raspberries. Efforts to understand the pathogenesis and epidemiology of Cyclospora have been limited by the inability of investigators to grow the organism in the laboratory. Little is known about modes of transmission or potential reservoirs of infection, resulting in the lack of adequate approaches for prevention and control of outbreaks. Investigators at the University of Arizona have the potential to develop more information about Cyclospora because of their association with the Cyclospora epidemiology project with the ICIDR site in Peru, and their access to numerous epidemiologically characterized isolates. The investigators plan to apply the techniques of PCR amplification of DNA from small numbers of organisms to the development of a sequence-based approach to the molecular epidemiology of Cyclospora infections. The ITS1 region between the small subunit ribosomal (r)DNA and 5.8S rDNA genes will be amplified, sequenced, and compared for a variety of epidemiologically characterized isolates. These isolates will be obtained from severely ill and asymptomatic patients, human immunodeficiency (HIV)-infected patients and from the 1996 U.S. outbreak. The technique then can be applied to prospective studies of the epidemiology of Cyclospora infections.

描述（改编自申请摘要）：环孢子虫是 一个新认识的地方性和流行性腹泻的原因， 世界 当这种生物于1985年首次被发现时，它被认为是一种 原生动物（称为大隐藻）或类蓝细菌体（CLB）或 蓝色绿色荧光，因为它的自发荧光。 该微生物 1993年在亚利桑那大学被证实是一种球虫， 诱导形成孢子。 从那时起，环孢子虫继续被认为是 在许多发展中国家， 国家 它也被认为是一种流行病的原因， 例如，1996年有一次广为人知的爆发， 佛罗里达和其他13个州，可能与进口树莓有关。 为了解环孢子虫的发病机制和流行病学所做的努力， 由于研究人员无法在 实验室 对传播方式或潜在的 感染的水库，导致缺乏足够的方法， 预防和控制疫情。 大学的研究人员 亚利桑那州有潜力开发更多关于环孢子虫的信息 因为它们与环孢子虫流行病学项目有关， 在秘鲁的ICIDR站点，以及它们获得许多流行病学资料的途径， 特征分离物。 研究人员计划应用以下技术 PCR扩增DNA从少量的生物体发展到 环孢子虫的分子流行病学的基于序列的方法 感染. 小亚基核糖体（r）DNA和 5.8S rDNA基因将被扩增，测序，并比较各种 流行病学特征的分离物。 将获得这些分离株 来自重症和无症状患者，人类免疫缺陷 （艾滋病毒）感染的患者和1996年美国爆发。 该技术然后 可应用于环孢子虫流行病学的前瞻性研究 感染.